Abstract: A container assembly includes a container at least partially bounding a chamber and having a wall with an opening extending therethrough. A tubular stem bounds a passageway and outwardly projects from the wall of the container so that the passageway communicates with the opening. A sparger includes a tubular member having a first end and an opposing second end and an interior surface bounding a passage extending therebetween. A gas permeable sparging member is secured to the first end of the tubular member so that gas passing through the tubular member from the second end passes out through the sparging sheet. A second flange encircles and radially outwardly projects from the tubular member, the second flange being secured to the stem so that at least a portion of the tubular member is disposed within the passageway of the tubular stem.

Abstract: A system for sterilizing a fluid includes a sterilization container bounding a fluid flow path that can be coupled with a fluid source. The sterilization container is comprised of one or more polymeric walls. A beam generator is configured to direct a beam of electromagnetic radiation through at least a portion of the fluid flow path of the sterilization container, the beam of electromagnetic radiation being sufficient to sterilize a fluid within the fluid flow path.

Abstract: A heat exchanger system includes a heat exchanger having a body with a first side face and an opposing second side face, the body bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port. A flexible first bag has of one or more sheet of polymeric material and bounds a fluid pathway that extends between a fluid inlet and a fluid outlet. The first bag is removably retained against the first side face of the body of the heat exchanger, the fluid channel of the body of the heat exchanger being sealed from fluid communication with the fluid pathway of the first bag.

Abstract: A method for filtering a gas includes sparging a gas through a liquid within a compartment of a container. In one embodiment the container can comprise a flexible bag. The sparged gas is passed from the container through a gas filter of a filter assembly. A partial vacuum is applied to the gas filter so that the partial vacuum assists in drawing the gas through the gas filter.

Abstract: A system for sterilizing a fluid includes a fluid source and a sterilization container bounding a fluid flow path that is in fluid communication with the fluid source. The sterilization container is comprised of one or more polymeric walls. An electron beam generator is configured to direct an electron beam through at least a portion of the fluid flow path of the sterilization container, the electron beam being sufficient to sterilize a fluid within the fluid flow path.

Abstract: A heat exchanger system includes a heat exchanger having a body plate with a first side face and an opposing second side face, the body plate bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port, and a first side plate having an inside face. A flexible first bag includes of one or more sheets of polymeric material, the first bag bounding a fluid pathway that extends between a fluid inlet and a fluid outlet, the first bag being removably retained between the body plate and the first side plate so that at least a portion of the first bag rests against the first side face of the body plate when the fluid pathway of the first bag is filled with a fluid.

Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. A tubular port or tube is secured with the bag or the sparging sheet so that a passage bounded by the tubular port or tube communicates with the compartment.

Abstract: A filter assembly includes a casing having of a polymeric film and bounding a compartment, the casing having an inlet opening communicating with the compartment and a first outlet opening communicating with the compartment. A first filter includes a porous filter body through which gas can pass and has a pore size smaller than 1 ?m, the first filter being coupled with the casing so that the filter body is at least partially disposed within compartment of the casing and so that gas passing through the compartment of the casing from the inlet opening to the first outlet opening must pass through the filter body.

Abstract: A method for processing a fluid includes sparging a fluid within the compartment of a container with an initial gas so that the initial gas passes through a portion of the fluid to form a humid gas within the compartment. The humid gas is passed out of the compartment of the container and into a flexible condenser bag. The humid gas within the condenser bag is cooled so as to separate the humid gas within the condenser bag into a condensed fluid and a dehumidified gas. The condensed fluid and the dehumidified gas is then removed from the condenser bag.

Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. A tubular port or tube is secured with the bag or the sparging sheet so that a passage bounded by the tubular port or tube communicates with the compartment.

Abstract: A fluid mixing system that can be used as a bioreactor or fermentor can include a stand and a support housing pivotably mounted to the stand. The support housing bounds a chamber into which a container assembly is removably disposed. The container assembly includes a flexible bag bounding a compartment adapted to hold a fluid and a mixing element disposed within the compartment. The support housing can be tilted relative to the stand for ease in insertion of the container assembly. The support housing can also be repeatedly rocked relative to the stand form mixing fluid within the container assembly.

Abstract: A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. In one embodiment the liquid is a culture that includes cells or microorganisms and the mass transfer functions to oxygenate the culture sufficient to sustain the cells or microorganisms.

Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.

Abstract: A system for sterilizing a fluid includes a fluid source and a sterilization container bounding a fluid flow path that is in fluid communication with the fluid source. The sterilization container is comprised of one or more polymeric walls. An electron beam generator is configured to direct an electron beam through at least a portion of the fluid flow path of the sterilization container, the electron beam being sufficient to sterilize a fluid within the fluid flow path.

Abstract: A heat exchanger system includes a heat exchanger having a body plate with a first side face and an opposing second side face, the body plate bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port, and a first side plate having an inside face. A flexible first bag includes of one or more sheets of polymeric material, the first bag bounding a fluid pathway that extends between a fluid inlet and a fluid outlet, the first bag being removably retained between the body plate and the first side plate so that at least a portion of the first bag rests against the first side face of the body plate when the fluid pathway of the first bag is filled with a fluid.

Abstract: A method of mixing a fluid includes positioning a flexible bag into the chamber of a support housing, the support housing having a plurality of baffles positioned so that the bag folds around the baffles. A fluid is delivered into a compartment of the flexible bag. A mixing element, such as an impeller, is moved within the compartment of the flexible bag so as to mix the fluid therein.

Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.

Abstract: A reactor system includes a flexible bag having an interior surface bounding a compartment. An elongated tubular connector is disposed within the compartment of the flexible bag, the tubular connector having a first end and an opposing second end, the first end of the tubular connector being coupled with the flexible bag. An impeller is disposed within the compartment of the flexible bag and is secured to the tubular connector. A drive shaft is removably received within the tubular connector such that rotation of the drive shaft facilitates rotation of the impeller.

Abstract: A sparger includes a body bounding a compartment. A tubular port or tube is coupled with the body. The tubular port or tub bounds a passage that communicates with the compartment of the body. At least a portion of the body includes of a first sparging sheet. The first sparging sheet includes a flexible sheet of a gas permeable material such that a gas passing into the compartment of the body through the passage can exit the compartment by permeating through the first sparging sheet.

Abstract: A method for processing a fluid includes sparging a fluid within the compartment of a container with an initial gas so that the initial gas passes through a portion of the fluid to form a humid gas within the compartment. The humid gas is passed out of the compartment of the container and into a flexible condenser bag. The humid gas within the condenser bag is cooled so as to separate the humid gas within the condenser bag into a condensed fluid and a dehumidified gas. The condensed fluid and the dehumidified gas is then removed from the condenser bag.