Abstract: Described herein are methods for the controlled de novo synthesis of RNA oligonucleotides using enzymatic catalysis. For example, provided herein are methods for preparing RNA oligonucleotides via controlled, template-independent addition of nucleotides to an initiator oligonucleotide 3?-terminus via enzymatic catalysis (also known as terminal transferase activity). Single nucleotides can be iteratively added by a compatible polymerase (e.g., a poly(N) polymerase such as a poly(U) polymerase) until a desired RNA oligonucleotide sequence is synthesized. Also provided are nucleotides and polymerases useful in the methods described herein.

Abstract: An electrical control system for a rotatable wheel cover assembly is presented, the system including a controller assembly comprising processors for controlling the relative motion of a wheel cover rotatably mounted to a wheel frame of a vehicle. The controller assembly outputs a first signal to motor drivers, which are mounted within the vehicle separated from the wheel cover, the motor drivers passing a first drive frequency to motor leads for open loop control of the speed of the motors that drive the wheel cover. The electrical control system provides power from an inboard side of the wheel frame to an outboard side via a slip ring assembly having connector assemblies passing through the wheel frame for controlling motion of the wheel cover. The wheel cover is mounted to a shaft of the motor, wherein the motor is mounted coaxially with the wheel frame.

Abstract: An electrical control system for a rotatable wheel cover assembly is presented, the system including a controller assembly comprising processors for controlling the relative motion of a wheel cover rotatably mounted to a wheel frame of a vehicle. The controller assembly outputs a first signal to motor drivers, which are mounted within the vehicle separated from the wheel cover, the motor drivers passing a first drive frequency to motor leads for open loop control of the speed of the motors that drive the wheel cover. The electrical control system provides power from an inboard side of the wheel frame to an outboard side via a slip ring assembly having connector assemblies passing through the wheel frame for controlling motion of the wheel cover. The wheel cover is mounted to a shaft of the motor, wherein the motor is mounted coaxially with the wheel frame.

Abstract: An electrical control system for a rotatable wheel cover assembly is presented, the system including a controller assembly comprising processors for controlling the relative motion of a wheel cover rotatably mounted to a wheel frame of a vehicle. The controller assembly outputs a first signal to motor drivers, which are mounted within the vehicle separated from the wheel cover, the motor drivers passing a first drive frequency to motor leads for open loop control of the speed of the motors that drive the wheel cover. The electrical control system provides power from an inboard side of the wheel frame to an outboard side via a slip ring assembly having connector assemblies passing through the wheel frame for controlling motion of the wheel cover. The wheel cover is mounted to a shaft of the motor, wherein the motor is mounted coaxially with the wheel frame.