Abstract: Provided herein are methods of purifying messenger RNA (mRNA) by subjecting a preparation comprising in vitro synthesized mRNA to one or more steps of enzymatic digestion with a proteinase, optionally with a further oligo dT affinity chromatography step. Also provided are mRNA purified by the methods described herein.

Abstract: A tie placement apparatus having a body with a handgrip. The tie placement apparatus has a wire inlet adapted to receive a tie wire, a tie placement head, and an electric motor cooperative with the tie placement head so as to actuate the tie placement head. A first reel-holder is adapted to receive a tie wire reserve therein. The body has an attachment socket that removably receives the first reel holder. The attachment socket and the first reel holder are affixed together by an elastic latch.

Abstract: Disclosed is an apparatus for placing tarpaulins, having an apparatus body provided with a handle, and including: a wire inlet for receiving a fastening wire, a head for placing fasteners, at least one electric motor for actuating the placing head, and at least one first reel-holder for receiving a reserve of fastening wire. The apparatus body has an attachment base, which can receive the first reel-holder, for removable attachment of the first reel-holder to the apparatus body, the attachment base and the first reel-holder including a resilient lock. The apparatus can be used in the fields of arboriculture and viticulture.

Abstract: Disclosed is an apparatus for placing wire fasteners. The apparatus includes: a wire guide, a pivoting hook forming a section of the wire guide, a mechanical feeder of fastening wire, a blade for cutting the wire, a rotary twister, a single electric motor, a transmission mechanism having a wheel for sequential actuation of the hook and the blade, a first sensor of the angular position of the sequential actuation wheel, and an electronic circuit for controlling the electric motor connected to the first angular position sensor. The first angular position sensor is a continuously variable signal sensor that is sensitive to a rotation of the sequential actuation wheel over an angular range of 360 degrees. The apparatus can be used in the field of viticulture and arboriculture.

Abstract: The subject matter of the present invention is a novel vector and the use thereof for producing a heterologous protein or a gene of interest, that can be used, for example, in the context of an immunization or gene therapy programme and concerns in particular a self-replicating vector lacking an antibiotic-resistance gene, comprising a sequence encoding the ccdA protein functionally linked to a first promoter, the sequence of the Cer locus and a heterologous sequence, functionally linked to a second promoter.