Abstract: Methods for detection of a target nucleic acid in a sample are provided according to aspects of the present disclosure which include: providing a reaction mixture comprising a LAMP assay primer set specific for the target nucleic acid, magnesium, dNTPs, a reaction buffer, a DNA polymerase, and a sample to be tested for presence of the target nucleic acid, wherein the LAMP assay primer set comprises a forward inner primer (FIP), a backward inner primer (BIP), a forward outer primer (F3) and a backward outer primer (B3), wherein the FIP and BIP are present in a non-equal ratio such that molar concentration of BIP is highly skewed relative to molar concentration of FIP. The reaction mixture is incubated under amplification reaction conditions to produce a reaction product comprising amplified target nucleic acids. The amplified target nucleic acids are then detected.

Abstract: Polyaromatic hydrocarbons (PAH) from industrial waste and oil spills are a recurrent threat to food safety due to their stability and toxicity. Existing methods to establish the concentrations of PAH in environmental and food samples rely on expensive and laborious methodology. The teachings herein provide methods and kits for detecting the presence or absence of PAH. In certain embodiments, the quantity of PAH in a sample can be determined.

Abstract: Provided herein, inter alia, are methods and compositions for removing a phosphopantethiene analog moiety from an ACP-phosphopantetheine conjugate thereby providing Apo-ACP proteins.

Abstract: Polyaromatic hydrocarbons (PAH) from industrial waste and oil spills are a recurrent threat to food safety due to their stability and toxicity. Existing methods to establish the concentrations of PAH in environmental and food samples rely on expensive and laborious methodology. The teachings herein provide methods and kits for detecting the presence or absence of PAH. In certain embodiments, the quantity of PAH in a sample can be determined.

Abstract: Provided herein, inter alia, are methods and compositions for removing a phosphopantethiene analogue moiety from an ACP-phosphopantetheine conjugate thereby providing Apo-ACP proteins.