Patents by Inventor Nicole L. Arnold
Nicole L. Arnold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200385752Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes areas selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: August 20, 2020Publication date: December 10, 2020Applicant: Dow AgroSciences LLCInventors: Justin M Lira, Terry Wright, Andrew E. Robinson, Sean Russell, Donald J Merlo, Steven Webb, Nicole L Arnold, Kelley E Smith
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Publication number: 20200385751Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: August 20, 2020Publication date: December 10, 2020Applicant: Dow AgroSciences LLCInventors: Justin M Lira, Terry Wright, Andrew E. Robinson, Sean Russell, Donald J Merlo, Steven Webb, Nicole L Arnold, Kelley E Smith
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Patent number: 10752913Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: GrantFiled: December 7, 2007Date of Patent: August 25, 2020Assignee: Dow AgroSciences LLCInventors: Justin M. Lira, Terry R. Wright, Sean M. Russell, Donald J. Merlo, Steven R. Webb, Nicole L. Arnold, Andrew E. Robinson, Kelley A. Smith
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Patent number: 10662434Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: November 12, 2015Date of Patent: May 26, 2020Assignees: Dow AgroSciences LLC, Sangamo Therapeutics, Inc.Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey C. Miller, Fyodor Urnov
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Patent number: 9402358Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.Type: GrantFiled: August 18, 2010Date of Patent: August 2, 2016Assignee: Dow AgroSciences LLCInventors: Yunxing C. Cui, Jill R. Bryan, Donald G. Maum, Gregory J. Gilles, Terry R. Wright, Jennifer L. Hamilton, Nicole L. Arnold, Nathan J. VanOpdorp, Tina M. Kaiser, Ning Zhou
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Publication number: 20160130314Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: ApplicationFiled: November 12, 2015Publication date: May 12, 2016Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey C. Miller, Fyodor Urnov
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Patent number: 9187758Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: December 13, 2007Date of Patent: November 17, 2015Assignees: Sangamo BioSciences, Inc., Dow AgroSciences LLCInventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
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Patent number: 9127289Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.Type: GrantFiled: November 22, 2010Date of Patent: September 8, 2015Assignee: Dow AgroSciences LLCInventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
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Patent number: 8921112Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: October 1, 2008Date of Patent: December 30, 2014Assignees: Dow AgroSciences LLC, Sangamo BioSciences, Inc.Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
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Patent number: 8598413Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided. The subject invention also includes related methods of controlling weeds.Type: GrantFiled: February 22, 2011Date of Patent: December 3, 2013Assignee: Dow AgroSciecnes, LLC.Inventors: Yunxing Cui, Jill Rene Bryan, Donald Glynn Maum, Terry Wright, Jennifer L. Hamilton, Nicole L Arnold, Nathan VanOpdorp, Tina Marie Kaiser, Ning Zhou, Steve Allan McMaster, Mark Allen Peterson, Leon Bo Braxton, Gregory J. Gilles
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Publication number: 20120222153Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.Type: ApplicationFiled: August 18, 2010Publication date: August 30, 2012Applicant: DOW AGROSCIENCES LLCInventors: Yunxing C. Cui, Jill R. Bryan, Donald G. Maum, Gregory J. Gilles, Terry R. Wright, Jennifer L. Hamilton, Nicole L. Arnold, Nathan J. VanOpdorp, Tina M. Kaiser, Ning Zhou
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Publication number: 20110289620Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided. The subject invention also includes related methods of controlling weeds.Type: ApplicationFiled: February 22, 2011Publication date: November 24, 2011Applicant: Dow AgroSciences LLCInventors: Yunxing Cui, Jill Rene Bryan, Donald Glynn Maum, Terry Wright, Jennifer L. Hamilton, Nicole L. Arnold, Nathan Vanopdorp, Tina Marie Kaiser, Ning Zhou, Steve Allan McMaster, Mark Allen Peterson, Leon Bo Braxton, Gregory J. Gilles
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Publication number: 20110124503Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.Type: ApplicationFiled: November 22, 2010Publication date: May 26, 2011Applicant: Dow AgroSciences LLCInventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
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Publication number: 20110107455Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: December 7, 2007Publication date: May 5, 2011Applicant: Dow AgroSciences LLCInventors: Justin M. Lira, Terry R. Wright, Sean M. Russell, Donald J. Merlo, Steven Robert Webb, Nicole L. Arnold, Andrew E. Robinson, Kelley A. Smith
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Publication number: 20090111188Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: ApplicationFiled: October 1, 2008Publication date: April 30, 2009Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
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Publication number: 20090093366Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.Type: ApplicationFiled: May 2, 2005Publication date: April 9, 2009Applicant: Dow AgroSciences LLCInventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
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Publication number: 20080182332Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: ApplicationFiled: December 13, 2007Publication date: July 31, 2008Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov