Abstract: The present disclosure relates to glycoprotein CD52 and fusion proteins thereof, wherein the CD52 glycoprotein has ?-2,3-sialylated N-glycans, O-glycosylation and a pI of about 5 to about 6. The disclosure further relates to the preparation and purification of these proteins and their use in the suppression of effector T-cell function and/or immune response, such as in the treatment of diseases or conditions mediated by effector T-cell function.

Abstract: The invention relates to methods for the production of mesenchymal stem cells (MSCs), in particular to methods for the large scale production of MSCs, such as allogeneic MSCs, for use in treating various diseases in humans and other animals. The invention also relates to methods which permit the selection of preferred donor cells suitable for large seal e production of MSCs. The invention also relates to purified MSCs prepared by the methods of the invention. The invention also relates to the use of plate let lysate in methods for preparing cultures of MSCs and to the preparation of extracellular matrix-enriched secretions. The invention also relates to methods for the preparation of compositions comprising one or more component(s) secreted from cultured MSCs, having improved stability characteristics.

Abstract: The invention relates to methods for the production of mesenchymal stem cells (MSCs), in particular to methods for the large scale production of MSCs, such as allogeneic MSCs, for use in treating various diseases in humans and other animals. The invention also relates to methods which permit the selection of preferred donor cells suitable for l arge scal e production of MSCs. The invention also relates to purified MSCs prepared by the methods of the invention. The invention also relates to the use of platelet lysate in methods for preparing cultures of MSCs and to the preparation of extracellular matrix-enriched secretions. The invention also relates to methods for the preparation of compositions comprising one or more component(s) secreted from cultured MSCs, having improved stability characteristics.

Abstract: Described is a method for analysing at least one sample in an array of samples, the method including recording an image of the position of at least one sample relative to the other samples; utilising the recorded image to allow the application of a reagent or a succession of reagents to at least one sample in situ; and analysing at least one sample for a reaction with the reagent(s). Also described is an apparatus for analysing at least one sample in an array of samples, the apparatus including (a) a device to record an image of the position of at least one sample relative to the other samples; (b) a device to apply a reagent or sequence of reagents to at least one sample in situ; (c) a device to analyse at least one sample for a reaction with the reagent(s); and (d) a device to control the application of reagent(s) to the samples.

Abstract: Described is a method for analyzing at least one sample in an array of samples, the method including recording an image of the position of at least one sample relative to the other sample; utilizing the recorded image to allow the application of a reagent or a succession of reagents to at least one sample in situ; and analyzing at least one sample for a reaction with the reagent(s). Also described is an apparatus for analyzing at least one sample in an array of samples, the apparatus including (a) a device to record an image of the position of at least one sample relative to the other samples; (b) a device to apply a reagent or sequence of reagents to at least one sample in situ; (c) a device to analyse at least one sample for a reaction with the reagent(s); and (d) a device to control the application of reagent(s) to the samples.

Abstract: The present invention provides a method of recovering O-linked oligosaccharides from a macromolecule, the method comprising the steps: exposing the macromolecule to an alkaline agent to release O-linked olisaccharides from the macromolecule; separating the released oligosaccharide from the macromolecule; and recovering the oligosaccharide.

Abstract: An electrophoresis apparatus for the simultaneous electrophoretic separation of a plurality of component mixtures using a disposable tray having a plurality of parallel troughs shaped to retain an elongate strip of isoelectric focusing medium and sufficient liquid to immerse the elongate strip. A frame is provided to retain the tray in a horizontal position using a pair of rotatably mounted support members moving between an open and closed position. Support members are also connected to a plurality of electrodes aligned with each of the troughs so that the electrodes are positioned clear of the troughs in the open position and positioned in the troughs when in the closed position.

Abstract: Methods for producing glycan nitrones comprising reacting glycans with a mono-N-substituted hydroxylamine at pH 6 or greater to form glycan nitrones. Methods for producing glycan derivatives comprising reacting glycan nitrones produced by reacting glycans with a mono-N-substituted hydroxylamine at pH 6 or greater with a reducing agent to form hydroxylamine glycan derivatives and uses of the glycan nitrones and hydroxylamine glycan derivatives produced by the methods.

Abstract: A preparative method of separating oligosaccharides from contaminants such as peptides and salts, comprising the steps of reacting a solution containing oligosaccharides and contaminants with a solid support comprising graphitized carbon such that the oligosaccharides substantially bind to the solid support; washing the support to remove any contaminants not bound to the solid support; and eluting the bound oligosaccharide from the support without eluting bound contaminants to obtain a solution of oligosaccharides substantially free of contaminants.

Abstract: The present invention consists in methods of preparing derivatives either isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilising reducing sugars onto solid supports and methods to obtain sugars from glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid-phase Edman degradation followed by separating and characterizing the sugars.

Abstract: The present invention consists in methods of preparing sugar derivatives either in isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilizing reducing sugars onto solid supports and methods to obtain sugars from a glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid phase Edman degradation followed by separating and characterizing the sugars.