Abstract: Provided is a modified antibody which enables the quantitative measurement of the amount of a heparin/PF4 complex, an onset factor of heparin-induced trombocytopenia (HIT), without the influence of the presence of PF4, and which can be used as an HIT antibody standard specific for the heparin/PF4 complex. The modified antibody is prepared by linking a human IgG, or an antibody fragment derived from a human IgG, to a monoclonal antibody obtained by immunizing an animal (excluding a human) with the heparin/PF4 complex.

Abstract: Provided is a modified antibody which enables the quantitative measurement of the amount of a heparin/PF4 complex, an onset factor of heparin-induced trombocytopenia (HIT), without the influence of the presence of PF4, and which can be used as an HIT antibody standard specific for the heparin/PF4 complex. The modified antibody is prepared by linking a human IgG, or an antibody fragment derived from a human IgG, to a monoclonal antibody obtained by immunizing an animal (excluding a human) with the heparin/PF4 complex.

Abstract: A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of (1) a core portion substantially made of a water-insoluble polymer compound; and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer, characterized in that the signal-generating substance is included in the core portion, is disclosed, and a process for producing the same is also disclosed. The core-shell particle including a signal-generating substance therein is useful as a labeling substance in which neither radioisotopes having handling restrictions nor unstable enzymes are required, a high sensitivity superior to fluorescent substances is achieved, and nonspecific absorption does not occur.

Abstract: Provided are novel complexes containing a crosslinked avidin, an analyzing method and analyzing reagents and kits whereby a compound to be analyzed can be quickly, conveniently and accurately analyzed while taking advantage of the avidin-biotin reaction. The complexes contain at least two homogeneous or heterogeneous biotin-introduced products and one crosslinked avidin sandwiched therebetween. In the analyzing method, the homogeneous or heterogeneous biotin-introduced products and the crosslinked avidin are used. The analyzing reagent contains the crosslinked avidin. The analyzing kit contains the crosslinked avidin and a biotinylating agent.

Abstract: A method of bonding a substance to be incorporated into a free terminal of a water-soluble polymer compound chain, characterized by reacting a reactive functional group present at the free terminal of the water-soluble polymer compound chain which is bonded at a binding terminal thereof in the manner of bristles of a brush onto a surface of a base material; with the substance to be incorporated capable of reacting with the reactive functional group; in the presence of a water-soluble polymer compound which promotes the bonding, is disclosed.

Abstract: A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of (1) a core portion substantially made of a water-insoluble polymer compound; and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer, characterized in that the signal-generating substance is included in the core portion, is disclosed, and a process for producing the same is also disclosed.

Abstract: This invention relates to a method for immunologically measuring human thymidylate synthase with an anti-human thymidylate synthase antibody, wherein as the antibody, at least an anti-human thymidylate synthase polyclonal antibody immobilized on an insoluble matrix is used; a method for evaluating sensitivity of cancer cells to a fluoropyrimidine antitumor drug from the results of the measurement by the measuring method; and a human thymidylate synthase measuring kit comprising an insoluble matrix with at least one anti-human thymidylate synthase polyclonal antibody immobilized thereon. By a simple immunological measuring method, human TS in a sample can be measured with high sensitivity.

Abstract: A method for quantitatively measuring analyte in an undiluted whole blood sample is disclosed which involves contacting an undiluted whole blood sample comprising the analyte with magnetically-responsive particles which comprise a first partner which is coated on a magnetically-responsive particulate insoluble carrier, wherein the first partner specifically binds to the analyte to form a first complex of insoluble carrier-first partner-analyte, separating the resulting first complex from the undiluted whole blood sample, thereafter contacting the resulting separated first complex with a second partner which is labelled with a detectable marker and which specifically binds to the analyte to form a second complex of insoluble carrier-first partner-analyte-second partner, and separating the resulting second complex from unbound second partner by washing, and thereafter detecting the marker in the second complex to quantitatively measure the analyte in the undiluted whole blood sample, and wherein the initial con

Abstract: A quantitative determination of a substance is performed in a homogeneous system based on a change in enzyme activity; differences between the activity of a free enzyme and that of an enzyme bound by an aggregation or chemical bonding are observed. A peroxidase-labeled antibody and antigen system is one of of the typical example. Since the reaction is effected in a homogeneous system, the amount of antigen can be easily measured by the difference of enzyme activity.