Patents by Inventor Nobuyoshi Esaki
Nobuyoshi Esaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7452704Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.Type: GrantFiled: May 23, 2006Date of Patent: November 18, 2008Assignee: Mitsubishi Chemical CorporationInventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
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Publication number: 20070117191Abstract: The present invention relates to: a reductase gene for an ?-substituted-?,?-unsaturated carbonyl compound which contains a DNA sequence encoding an amino acid sequence represented by SEQ ID NO: 20 and an amino acid sequence represented by SEQ ID NO: 21; an enzyme which is a product of the gene; a plasmid and a transformant each containing the gene DNA; and a method of reducing an ?-substituted-?,?-unsaturated carbonyl compound using the transformant. According to claim the present invention, there is provided an enzyme gene which is useful in producing a corresponding ?-substituted-?,?-saturated carbonyl compound optically active at the ?-position by hydrogenating an ?,?-carbon double bond of an ?-substituted carbonyl compound, which is a compound prochiral at the alpha-position, and an enzyme which is a gene product thereof.Type: ApplicationFiled: February 6, 2003Publication date: May 24, 2007Inventors: Harumi Kamachi, Nobuyoshi Esaki, Tatsuo Kurihara
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Patent number: 7135306Abstract: A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.Type: GrantFiled: January 28, 2004Date of Patent: November 14, 2006Assignee: Alfresa Pharma CorproationInventors: Nobuyoshi Esaki, Tohru Yoshimura, Naoto Matsuyama, Koji Mizuno, Takayuki Bogaki, Toshitaka Minetoki, Kenji Ozeki
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Patent number: 7115735Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30 ° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.Type: GrantFiled: August 27, 2004Date of Patent: October 3, 2006Assignee: Mitsubishi Chemical CorporationInventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
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Publication number: 20060205045Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.Type: ApplicationFiled: May 23, 2006Publication date: September 14, 2006Applicant: Mitsubishi Chemical CorporationInventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
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Publication number: 20060166300Abstract: A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.Type: ApplicationFiled: January 28, 2004Publication date: July 27, 2006Applicant: ALFRESA PHARMA CORPORATIONInventors: Nobuyoshi Esaki, Tohru Yoshimura, Naoto Matsuyama, Koji Mizuno, Takayuki Bogaki, Toshitaka Minetoki, Kenji Ozeki
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Publication number: 20050124040Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.Type: ApplicationFiled: August 27, 2004Publication date: June 9, 2005Applicant: Mitsubishi Chemical CorporationInventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
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Patent number: 6645752Abstract: A method of producing an &agr;-halo-&agr;,&bgr;-saturated carbonyl compound from an &agr;-halocarbonyl compound having an &agr;,&bgr;-carbon-carbon double bond by reducing said &agr;,&bgr;-carbon-carbon double bond using a microorganism belonging to any one of the genera Acetobacter, Actinomyces, Acinetobacter, Agrobacterium, Aeromonas, Alcaligenes, Arthrobacter, Azotobacter, Bacillus, Brevibacterium, Burkholderia, Cellulomonas, Corynebacterium, Enterobacter, Enterococcus, Escherichia, Flavobacterium, Gluconobacter, Halobacteium, Halococccus, Klebsiella, Lactobacillus, Microbacterium, Micrococcus, Micropolyspora, Mycobacterium, Nocardia, Pseudomonas, Pseudonocardia, Rhodococcus, Rhodobacter, Serratia, Staphylococcus, Streptococcus and Streptomyces, Xanthomonas, or a microbial product thereof. Pseudomonas sp. SD810, SD811 and SD812, Burkholderia sp.Type: GrantFiled: September 26, 2001Date of Patent: November 11, 2003Assignee: Showa Denko Kabushiki KaishaInventors: Harumi Kamachi, Tadashi Yoneda, Motoaki Kamachi, Nobuyoshi Esaki
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Patent number: 6379935Abstract: A method of producing an &agr;-halo-&agr;,&bgr;-saturated carbonyl compound from an &agr;-halocarbonyl compound having an &agr;, &bgr;-carbon-carbon double bond by reducing said &agr;,&bgr;-carbon-carbon double bond using a microorganism belonging to any one of the genera Acetobacter, Actinomyces, Acinetobacter, Agrobacterium, Aeromonas, Alcaligenes, Arthrobacter, Azotobacter, Bacillus, Brevibacterium, Burkholderia, Cellulomonas, Corynebacterium, Enterobacter, Enterococcus, Escherichia, Flavobacterium, Gluconobacter, Halobacteium, Halococccus, Klebsiella, Lactobacillus, Microbacterium, Micrococcus, Micropolyspora, Mycobacterium, Nocardia, Pseudomonas, Pseudonocardia, Rhodococcus, Rhodobacter, Serratia, Staphylococcus, Streptococcus and Streptomyces, Xanthomonas, or a microbial product thereof. Pseudomonas sp. SD810, SD811 and SD812, Burkholderia sp.Type: GrantFiled: August 5, 1999Date of Patent: April 30, 2002Assignee: Showa Denko Kabushiki KaishaInventors: Harumi Kamachi, Tadashi Yoneda, Motoaki Kamachi, Nobuyoshi Esaki
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Publication number: 20020031811Abstract: A method of producing an &agr;-halo-&agr;,&bgr;-saturated carbonyl compound from an &agr;-halocarbonyl compound having an &agr;,&bgr;-carbon-carbon double bond by reducing said &agr;,&bgr;-carbon-carbon double bond using a microorganism belonging to any one of the genera Acetobacter, Actinomyces, Acinetobacter, Agrobacterium, Aeromonas, Alcaligenes, Arthrobacter, Azotobacter, Bacillus, Brevibacterium, Burkholderia, Cellulomonas, Corynebacterium, Enterobacter, Enterococcus, Escherichia, Flavobacterium, Gluconobacter, Halobacteium, Halococccus, Klebsiella, Lactobacillus, Microbacterium, Micrococcus, Micropolyspora, Mycobacterium, Nocardia, Pseudomonas, Pseudonocardia, Rhodococcus, Rhodobacter, Serratia, Staphylococcus, Streptococcus and Streptomyces, Xanthomonas, or a microbial product thereof. Pseudomonas sp. SD810, SD811 and SD812, Burkholderia sp.Type: ApplicationFiled: September 26, 2001Publication date: March 14, 2002Applicant: SHOWA DENKO K.K.Inventors: Harumi Kamachi, Tadashi Yoneda, Motoaki Kamachi, Nobuyoshi Esaki
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Publication number: 20020028491Abstract: A method of producing an &agr;-halo-&agr;,&bgr;-saturated carbonyl compound from an &agr;-halocarbonyl compound having an &agr;,&bgr;-carbon-carbon double bond by reducing said &agr;,&bgr;-carbon-carbon double bond using a microorganism belonging to any one of the genera Acetobacter, Actinomyces, Acinetobacter, Agrobacterium, Aeromonas, Alcaligenes, Arthrobacter, Azotobacter, Bacillus, Brevibacterium, Burkholderia, Cellulomonas, Corynebacterium, Enterobacter, Enterococcus, Escherichia, Flavobacterium, Gluconobacter, Halobacteium, Halococccus, Klebsiella, Lactobacillus, Microbacterium, Micrococcus, Micropolyspora, Mycobacterium, Nocardia, Pseudomonas, Pseudonocardia, Rhodococcus, Rhodobacter, Serratia, Staphylococcus, Streptococcus and Streptomyces, Xanthomonas, or a microbial product thereof. Pseudomonas sp. SD810, SD811 and SD812, Burkholderia sp.Type: ApplicationFiled: August 5, 1999Publication date: March 7, 2002Inventors: HARUMI KAMACHI, TADASHI YONEDA, MOTOAKI KAMACHI, NOBUYOSHI ESAKI
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Patent number: 5863788Abstract: A recombinant protein capable of constituting a recombinant L-methionine .gamma.-lyase or a variant thereof, a recombinant oligomeric enzyme, preferably tetrameric enzyme, consisting of the recombinant protein, a DNA molecule encoding the same, an expression vector containing the DNA molecule, a host cell harboring the expression vector, a method of preparing the recombinant L-methionine .gamma.-lyase, and anti-tumor agent comprising the recombinant L-methionine .gamma.-lyase or a variant thereof are provided.Type: GrantFiled: April 14, 1998Date of Patent: January 26, 1999Assignee: Shionogi & Co., Ltd.Inventors: Kenji Soda, Hidehiko Tanaka, Hiroyuki Inoue, Manabu Sugimoto, Kenji Inagaki, Nobuyoshi Esaki
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Patent number: 5861154Abstract: A recombinant protein capable of constituting a recombinant L-methionine .gamma.-lyase or a variant thereof, a recombinant oligomeric enzyme, preferably tetrameric enzyme, consisting of the recombinant protein, a DNA molecule encoding the same, an expression vector containing the DNA molecule, a host cell harboring the expression vector, a method of preparing the recombinant L-methionine .gamma.-lyase, and anti-tumor agent comprising the recombinant L-methionine .gamma.-lyase or a variant thereof are provided.Type: GrantFiled: April 30, 1996Date of Patent: January 19, 1999Assignee: Shionogi & Co., Ltd.Inventors: Kenji Soda, Hidehiko Tanaka, Hiroyuki Inoue, Manabu Sugimoto, Kenji Inagaki, Nobuyoshi Esaki
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Patent number: 5362636Abstract: A process for producing L-lysine, which comprises culturing a mutant L-lysine-producing strain belonging to the genus Brevibacterium or the genus Corynebacterium and having selenalysine resistance in a nutrient medium, producing and accumulating L-lysine in the culture broth and collecting L-lysine from the culture broth.Type: GrantFiled: October 22, 1991Date of Patent: November 8, 1994Assignee: Ajinomoto Co., Inc.Inventors: Manabu Yokomori, Takeshi Niwa, Kazuhiko Totsuka, Yoshio Kawahara, Shigeru Nakamori, Nobuyoshi Esaki, Kenji Soda