Abstract: The extract of Agathis robusta as an antifungal agent is prepared by hydrodistillation of the powdered bark of A. robusta in a Clevenger-type apparatus at 100° C. for 6 hours to obtain the essential oils of A. robusta. The essential oil is mixed with an ointment base of cholesterol, liquid paraffin, lanolin, and vaseline album, the powdered essential oil of A. robusta being added to the ointment base at 1 or 2% w/w and homogenized to a smooth ointment. When tested in vivo on Wistar rats topically infected with Candida albicans, both the 1% and 2% ointment formulations demonstrated substantial antifungal activity.