Abstract: A method of culturing cells including: dividing a culture area into a center area and a plurality of peripheral areas, and designating the center area and the plurality of peripheral areas as measurement positions; calculating: a confluent rate at each of the measurement positions designated; and an average confluent rate which is an average of sum of the confluent rates at the measurement positions designated, the confluent rate being defined as a proportion of an area occupied by cells in a designated area; and determining a timing to perform a subculture of the cells, based on the confluent rate, wherein the determining of the timing further includes determining when the average confluent rate is smaller than a first threshold value, the confluent rate of the center area is larger than a second threshold value, and the second threshold value is larger than the first threshold value.

Abstract: Cell culture device includes incubator unit that cultures cells in culture container, transport unit that transports culture container to incubator unit, task setting unit that sets a task relating to a culture of the cells, imaging unit serving as an example of a management information acquisition unit that acquires management information attached to culture container, and storage unit that stores the management information acquired by imaging unit. From storage unit, task setting unit reads the management information of plurality of culture containers serving as targets for carrying out the task, and causes display serving as an example of a display unit to display the management information.

Abstract: A pipette tip includes a main body section; and a straight pipe section disposed at the leading end of the main body section, in which the inner diameter of the straight pipe section is 0.8 mm to 1.5 mm inclusive, and the length of the straight pipe section is 5 mm to 15 mm inclusive.

Abstract: A method of culturing cells including: dividing a culture area into a center area and a plurality of peripheral areas, and designating the center area and the plurality of peripheral areas as measurement positions; calculating: a confluent rate at each of the measurement positions designated; and an average confluent rate which is an average of sum of the confluent rates at the measurement positions designated, the confluent rate being defined as a proportion of an area occupied by cells in a designated area; and determining a timing to perform a subculture of the cells, based on the confluent rate, wherein the determining of the timing further includes determining when the average confluent rate is smaller than a first threshold value, the confluent rate of the center area is larger than a second threshold value, and the second threshold value is larger than the first threshold value.

Abstract: A cell culture apparatus includes a measurement-position designating unit, an image processing unit, and a subculture-timing determining unit. The measurement-position designating unit designates measurement positions in the culture vessel. The image processing unit calculates a confluent rate as a proportion of an area occupied by the cells in the observation image, of the positions designated by the measurement-position designating unit. The subculture-timing determining unit determines the subculture timing of the culture vessel, based on the confluent rate calculated in the image processing unit. More specifically, the subculture-timing determining unit determines the subculture timing, based on a first threshold value with respect to an average value of the entire measurement area on the observation image, and a second threshold value with respect to at least one measurement area on the observation image. The second threshold value is larger than the first threshold value.

Abstract: An apparatus for culturing cells of the present invention is an apparatus for culturing cells using a culture medium in a culture vessel. The apparatus includes: pump connected to pipet held by first holder; first shifter configured to shift first holder; second holder configured to hold culture vessel; second shifter configured to grip and shift culture vessel; and controller. Controller is programmed to, when pipet draws a liquid in culture vessel or discharges a liquid into culture vessel, control second shifter to translationally shift lid of culture vessel in a horizontal direction.

Abstract: Cell culture device includes incubator unit that cultures cells in culture container, transport unit that transports culture container to incubator unit, task setting unit that sets a task relating to a culture of the cells, imaging unit serving as an example of a management information acquisition unit that acquires management information attached to culture container, and storage unit that stores the management information acquired by imaging unit. From storage unit, task setting unit reads the management information of plurality of culture containers serving as targets for carrying out the task, and causes display serving as an example of a display unit to display the management information.

Abstract: A liquid transferring apparatus sucks up or discharges liquid by moving piston of syringe vertically including first motor which actuates piston by supporting flange pulling-up portion to be vertically movable and by pulling up flange portion of piston from below via flange pulling-up portion; second motor which supports flange pushing portion to be vertically movable and actuates piston so as to push flange portion of piston from above via flange pushing portion; cylinder fixing portion which fixes cylinder of syringe; and mechanical frame.

Abstract: An apparatus for culturing cells of the present disclosure includes: a cabinet that has a main surface and a side surface, the main surface having a window; a main surface aspiration port disposed at the main surface of the cabinet; and a side surface aspiration port disposed at the side surface of the cabinet. In the cross section of the cabinet taken along the horizontal direction, a first vessel supplying part for supplying a first vessel is disposed at a midportion of the cabinet, and a second vessel supplying part for supplying a second vessel is disposed at an inner peripheral portion of the cabinet. The first vessel supplying part has a first lid capable of entirely covering the first vessel supplying part. The first vessel has no lid. The second vessel supplying part has no lid. The second vessel has a second lid.

Abstract: A cell culture apparatus includes a measurement-position designating unit, an image processing unit, and a subculture-timing determining unit. The measurement-position designating unit designates measurement positions in the culture vessel. The image processing unit calculates a confluent rate as a proportion of an area occupied by the cells in the observation image, of the positions designated by the measurement-position designating unit. The subculture-timing determining unit determines the subculture timing of the culture vessel, based on the confluent rate calculated in the image processing unit. More specifically, the subculture-timing determining unit determines the subculture timing, based on a first threshold value with respect to an average value of the entire measurement area on the observation image, and a second threshold value with respect to at least one measurement area on the observation image. The second threshold value is larger than the first threshold value.

Abstract: A pipette tip includes a main body section; and a straight pipe section disposed at the leading end of the main body section, in which the inner diameter of the straight pipe section is 0.8 mm to 1.5 mm inclusive, and the length of the straight pipe section is 5 mm to 15 mm inclusive.

Abstract: An apparatus for culturing cells using a culture medium, including: a replacer that replaces the culture medium in a culture vessel held by a holder; a measurer that measures a pH of the culture medium in the culture vessel; and an instructor that instructs the replacer to replace the culture medium in the culture vessel, wherein the instructor gives an instruction when the pH measured by the measurer is less than a predetermined pH threshold at a first replacement of the culture medium, and gives an instruction when the pH measured by the measurer is less than the predetermined pH threshold and the interval is less than an interval of a previous replacement of the culture medium at second and subsequent replacements of the culture medium.

Abstract: An apparatus for culturing cells of the present disclosure includes: a cabinet that has a main surface and a side surface, the main surface having a window; a main surface aspiration port disposed at the main surface of the cabinet; and a side surface aspiration port disposed at the side surface of the cabinet. In the cross section of the cabinet taken along the horizontal direction, a first vessel supplying part for supplying a first vessel is disposed at a midportion of the cabinet, and a second vessel supplying part for supplying a second vessel is disposed at an inner peripheral portion of the cabinet. The first vessel supplying part has a first lid capable of entirely covering the first vessel supplying part. The first vessel has no lid. The second vessel supplying part has no lid. The second vessel has a second lid.

Abstract: An apparatus for culturing cells of the present invention is an apparatus for culturing cells using a culture medium in a culture vessel. The apparatus includes: pump connected to pipet held by first holder; first shifter configured to shift first holder; second holder configured to hold culture vessel; second shifter configured to grip and shift culture vessel; and controller. Controller is programmed to, when pipet draws a liquid in culture vessel or discharges a liquid into culture vessel, control second shifter to translationally shift lid of culture vessel in a horizontal direction.

Abstract: An apparatus for culturing cells using a culture medium, including: a replacer that replaces the culture medium in a culture vessel held by a holder; a measurer that measures a pH of the culture medium in the culture vessel; and an instructor that instructs the replacer to replace the culture medium in the culture vessel, wherein the instructor gives an instruction when the pH measured by the measurer is less than a predetermined pH threshold at a first replacement of the culture medium, and gives an instruction when the pH measured by the measurer is less than the predetermined pH threshold and the interval is less than an interval of a previous replacement of the culture medium at second and subsequent replacements of the culture medium.