Abstract: A method of manufacturing a template, has: preparing a substrate containing quartz and having a surface, the surface including a protrusion and a depression; and processing the depression. The processing of the depression includes: a first step of forming a film on the surface, the film including a first region and a second region, the first region being provided on the protrusion, and the second region being provided on a bottom of the depression and being thinner than the first region; a second step of removing the second region with the first region partly remaining to expose the bottom of the depression; and a third step of processing the exposed part of the depression using a mask made of the remainder of the first region.

Abstract: It is an object of the present invention to provide a method for killing tumor cells, having a few side effects. The present invention provides a method for killing tumor cells, comprising: (1) a step of allowing an immunotoxin formed by binding an antibody binding to ROBO1 or a fragment thereof to a cytotoxin to come into contact with tumor cells; (2) a step of allowing a photosensitizer that induces photochemical cytoplasmic internalization to come into contact with the tumor cells; and (3) a step of irradiating the tumor cells with a wave length that is effective for activating the sensitizer, so as to kill the cells.

Abstract: A calcium-phosphate type hydroxyapatite for chromatographic separation is in the form of spherulites having a mean particle diameter of 0.5 to 50 .mu.m, preferably 1 to 10 .mu.m. The spherulites are each an aggregate mainly of acicular crystallites belonging to a series of hexagonal systems and having unit lattice constants of 9.58.+-.0.08 .ANG. for the a axis and 7.00.+-.0.05 .ANG. for the c axis. The hydroxyapatite is produced by granulating a calcium=phosphate type hydroxyapatite in the form of a gel and then firing the granular apatite at 400.degree. to 700.degree. C., preferably at 500.degree. to 600.degree. C. The firing is performed by heating the granules in the presence of oxygen or air for a given period of time.

Abstract: A calcium-phosphate type hydroxyapatite for chromatographic separation is in the form of spherulites having a mean particle diameter of 0.5 to 50 .mu.m, preferably 1 to 10 .mu.m. The spherulites are each an aggregate mainly of acicular crystallites belonging to a series of hexagonal systems and having unit lattice constants of 9.58.+-.0.08 .ANG. for the a axis and 7.00.+-.0.05 .ANG. for the c axis. The hydroxyapatite is produced by granulating a calcium-phosphate type hydroxyapatite in the form of a gel and then firing the granular apatite at 400.degree. to 700.degree. C., preferably at 500.degree. to 600.degree. C. The firing is performed by heating the granules in the presence of oxygen or air for a given period of time.

Abstract: An assemblage of hydroxyl apatite particles having small and uniform particle size. The median of the particle size is 1 to 10 .mu.m, and at least 90% of the particles in the assemblage have a particle size of not more than 25 .mu.m. The assemblage can be used to pack a liquid chromatography column.

Abstract: A cell separator which can separate cells, especially lymphocytes, with high selectivity and high efficiency without changing the cell population ratio of the cells. The cell separator comprises fibrous material containing fibers including hydroxyl apatite.

Abstract: A calcium-phosphate type hydroxyapatite which belongs to a series of hexagonal systems has unit lattice constants of 9.58.+-.0.08 .ANG. for the a axis and 7.00.+-.0.05 .ANG. for the c axis. Its Ca/P ratio is in the range of 1.50 to 1.90. It is for use as a column packing material for chromatographic separation of biopolymers. The hydroxyapatite is produced by firing it in the form of a gel or powder at a temperature of 400.degree. to 700.degree. C. As a gel the hydroxyapatite takes the form of a suspension or slurry. A powder is prepared by removing moisture from the hydroxyapatite in the gel form and then drying. Either gel or powder is fired by heating in the presence of oxygen or air at 500.degree. to 600.degree.0 C.

Abstract: A calcium-phosphate type hydroxyapatite for chromatographic separation is in the form of spherulites having a mean particle diameter of 0.5 to 50 .mu.m, preferably 1 to 10 .mu.m. The spherulites are each an aggregate mainly of acicular crystallites belonging to a series of hexagonal systems and having unit lattice constants of 9.58.+-.0.08 .ANG. for the a axis and 7.00.+-.0.05 .ANG. for the c axis. The hydroxyapatite is produced by granulating a calcium-phosphate type hydroxyapatite in the form of a gel and then firing the granular apatite at 400.degree. to 700.degree. C., preferably at 500.degree. to 600.degree. C. The firing is performed by heating the granules in the presence of oxygen or air for a given period of time.