Patents by Inventor Olga Petrauskene
Olga Petrauskene has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9850545Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.Type: GrantFiled: June 25, 2014Date of Patent: December 26, 2017Assignee: Life Technologies CorporationInventors: Olga Petrauskene, Pius Brzoska, Somaya Bit, Jen-Kuei Liu, Robert Tebbs, Manohar Furtado
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Patent number: 9834815Abstract: The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis.Type: GrantFiled: March 25, 2010Date of Patent: December 5, 2017Assignee: Life Technologies CorporationInventors: Michael Brewer, Olga Petrauskene, Jen-Kuei Liu, Craig Cummings, Sueh-Ning Liew
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Patent number: 9546405Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.Type: GrantFiled: June 19, 2014Date of Patent: January 17, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Olga Petrauskene, Craig Cummings, Paolo Vatta, Robert Tebbs, Priya Balachandran, Patrick Zoder, Lily Wong
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Detection of subspecies serovar in food and environmental samples, methods and compositions therefor
Patent number: 9175354Abstract: Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica, subspecies enterica, serovar Enteritidis (S. Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.Type: GrantFiled: August 3, 2011Date of Patent: November 3, 2015Assignee: Life Technologies CorporationInventors: Robert Tebbs, Craig Cummings, Arlene Nunez, Priya Balachandran, Payman Fatemi, Olga Petrauskene -
Publication number: 20150217293Abstract: A fluid processing device and methods are provided that can process one or many different fluid samples, detection for each of which can be multiplexed to detect the presence or absence of each of a panel of target sequences, for example 20 different target sequences. The device can comprise a substrate and one or more fluid processing pathways at least partially defined by the substrate. Each fluid processing pathway can comprise a pre-amplification region and two or more amplification regions disposed downstream from and in fluid communication with the pre-amplification region. A burstable valve can be disposed along each fluid processing pathway and the downstream regions can contain pre-loaded ammonia gas to draw an amplified sample downstream.Type: ApplicationFiled: February 6, 2015Publication date: August 6, 2015Inventors: Konrad Faulstich, Mark F. Oldham, Maxim G. Brevnov, Olga Petrauskene, Manohar R. Furtado
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Publication number: 20150203901Abstract: Described are compositions, methods and kits for detection and/or differential detection of serovars of Salmonella enterica subsp. enterica serovar such as S. Heidelberg and S. Hadar in samples. Some embodiments relate to multiplex amplification based molecular assays.Type: ApplicationFiled: April 6, 2015Publication date: July 23, 2015Inventors: Craig CUMMINGS, Olga Petrauskene, Lily Wong, Allison De Los Reyes
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Patent number: 9024002Abstract: Described are compositions, methods and kits for detection and/or differential detection of serovars of Salmonella enterica subsp. enterica serovar such as S. Heidelberg and S. Hadar in samples. Some embodiments relate to multiplex amplification based molecular assays.Type: GrantFiled: March 7, 2013Date of Patent: May 5, 2015Assignee: Life Technologies CorporationInventors: Craig Cummings, Olga Petrauskene, Lily Wong, Allison De Los Reyes
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Publication number: 20150056626Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.Type: ApplicationFiled: November 7, 2014Publication date: February 26, 2015Inventors: PAOLO VATTA, OLGA PETRAUSKENE, MANOHAR FURTADO, PIUS BRZOSKA, LILY WONG, MELISSA BARKER, CRAIG CUMMINGS
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Publication number: 20150045242Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.Type: ApplicationFiled: June 19, 2014Publication date: February 12, 2015Inventors: Olga PETRAUSKENE, Craig Cummings, Paolo Vatta, Robert Tebbs, Priya Balachandran, Patrick Zoder, Lily Wong
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Publication number: 20140378330Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.Type: ApplicationFiled: June 25, 2014Publication date: December 25, 2014Inventors: Olga PETRAUSKENE, Pius Brzoska, Somaya Bit, Jen-Kuei Liu, Robert Tebbs, Manohar Furtado
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Patent number: 8906628Abstract: Disclosed are methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.Type: GrantFiled: April 25, 2013Date of Patent: December 9, 2014Assignee: Life Technologies CorporationInventors: Paolo Vatta, Olga Petrauskene, Manohar Furtado, Pius Brzoska, Lily Wong, Melissa Barker, Craig Cummings
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Patent number: 8795969Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.Type: GrantFiled: June 30, 2011Date of Patent: August 5, 2014Assignee: Life Technologies CorporationInventors: Olga Petrauskene, Craig Cummings, Paolo Vatta, Robert Tebbs, Priya Balachandran, Patrick Zoder, Lily Wong
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Publication number: 20140080130Abstract: Described are compositions, methods and kits for detection and/or differential detection of serovars of Salmonella enterica subsp. enterica serovar such as S. Heidelberg and S. Hadar in samples. Some embodiments relate to multiplex amplification based molecular assays.Type: ApplicationFiled: March 7, 2013Publication date: March 20, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Craig CUMMINGS, Olga PETRAUSKENE, Lily WONG, Allison DE LOS REYES
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Publication number: 20130236896Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.Type: ApplicationFiled: April 25, 2013Publication date: September 12, 2013Applicant: Life Technologies CorporationInventors: Paolo VATTA, Olga Petrauskene, Manohar Furtado, Pius Brzoska, Lily Wong, Melissa Barker, Craig Cummings
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Patent number: 8445209Abstract: Disclosed are primer and probe compositions, PCR assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from a variety of samples. In some embodiments, methods for specifically detecting E. coli O157:H7 comprise: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying said at least first and said at least second target polynucleotide sequences, and detecting said at least first and said at least second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.Type: GrantFiled: May 14, 2010Date of Patent: May 21, 2013Assignee: LIfe Technologies CorporationInventors: Paolo Vatta, Olga Petrauskene, Manohar Furtado, Pius Brzoska, Lily Wong, Melissa Barker, Craig A. Cummings
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Publication number: 20130122505Abstract: The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.Type: ApplicationFiled: August 24, 2012Publication date: May 16, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Robert TEBBS, Craig Cummings, Pius Brzoska, Sharon Matheny, Catherine O'Connell, Manohar Furtado, Rixun Fang, Olga Petrauskene, Sueh-Ning Liew, Michael Schumaker
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Publication number: 20120034606Abstract: Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica, subspecies enterica, serovar Enteritidis (S. Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.Type: ApplicationFiled: August 3, 2011Publication date: February 9, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Robert TEBBS, Craig Cummings, Arlene Nunez, Priya Balachandran, Payman Fatemi, Olga Petrauskene
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Publication number: 20120009575Abstract: The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.Type: ApplicationFiled: June 30, 2011Publication date: January 12, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Priya Balachandran, Craig Cummings, Olga Petrauskene, Hemant Pawar, Robert Tebbs, Manohar Furtado
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Publication number: 20120009574Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.Type: ApplicationFiled: June 30, 2011Publication date: January 12, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Olga Petrauskene, Craig Cummings, Paolo Vatta, Robert Tebbs, Priya Balachandran, Patrick Zoder, Lily Wong
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Publication number: 20110111403Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.Type: ApplicationFiled: October 29, 2010Publication date: May 12, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Olga PETRAUSKENE, Pius Brzoska, Somaya Bit, Jen-Kuei Liu, Robert Tebbs, Manohar Furtado