Abstract: Described in this disclosure are CRISPRi systems and methods, along with the related compositions and kits, that combine modularity, stable genomic integration, and ease of transfer to diverse bacteria by conjugation. CRISPRi compositions, methods, systems and kits described herein allow for genetic dissection of bacteria, facilitating analyses of microbiome function, antibiotic resistances and sensitivities, as well as comprehensive screening for host-microbe interactions. Embodiments of the invention comprise compositions, methods, systems, and kits for CRISPRi-based repression of gene expression in bacteria.

Abstract: A wearable sensor belt used as a reference frame for determining a location of an in-vivo device in the gastrointestinal (GI) tract, the belt including N magnetic field generating coils and M magnetic field sensors configured for dynamic calibration of the belt's geometry in order to accommodate for dynamic changes in the shape and/or size of the belt from one subject to another, and for dynamic changes in the shape and/or size of the belt as a result of changes in a subject's posture. A method for localizing an in-vivo device swallowed by a subject using a sensor belt is also described.

Abstract: A control circuit for controlling a state of a switching circuit may include a first unit to sense and interpret a wireless signal or physical parameter as an “on” signal to transition the switching circuit to the “on” state, or as an “off” signal to transition the switching circuit to the “off” state, and to transfer a first digital signal or logic value and/or a second digital signal or logic value, which may respectively or combinatorially represent the “on” signal or the “off” signal, to a second unit via a first output and/or a second output of the first unit, respectively. The second unit may force a control input of the switching circuit to a logic value which is a function of the first digital signal or value and/or second digital signal or value and congruent with the state to which the switching circuit is to be transitioned.

Abstract: A swallowable in-vivo device contains a movement detection unit that includes a movement sensing unit, a frequency analyzing unit (FAU) and a time analyzing unit (TAU). The movement sensing unit senses movements of the in-vivo device relative to a non-stationary three-dimensional reference frame, and outputs a movement signal. The frequency analyzing unit may analyze the movement signal spectrally to detect a potential command-invoking movement, and the time analyzing unit may analyze the potential CIM temporally, possibly in conjunction with a series of other movement events, to determine whether the potential CIM is a genuine CIM. If the potential CIM is determined to be a genuine CIM, the in-vivo device may execute a predetermined command associated with the CIM. Otherwise, the in-vivo device may refrain from executing a CIM-related command. A PCB including the movement detection unit and a processor for processing their output is provided for the vivo sensing device.

Abstract: A wearable sensor belt used as a reference frame for determining a location of an in-vivo device in the gastrointestinal (GI) tract, the belt including N magnetic field generating coils and M magnetic field sensors configured for dynamic calibration of the belt's geometry in order to accommodate for dynamic changes in the shape and/or size of the belt from one subject to another, and for dynamic changes in the shape and/or size of the belt as a result of changes in a subject's posture. A method for localizing an in-vivo device swallowed by a subject using a sensor belt is also described.

Abstract: An in-vivo device may allocate a sensing window in a work cycle for sensing and processing localization signals to determine the location/orientation of the in-vivo device. The in-vivo device may use a timing unit to schedule transmission of data frames and the sensing of the localization signals relative to a reference time embedded in work cycles. The timing unit may produce a clock signal based on which the in-vivo device may measure time specifics, which define the sensing window, relative to the reference time. A receiver may use data embedded in data frames to restore the clock signal and the reference time, and, from them, and using identical or similar time specifics, generate a synchronization signal for a localization signals source (LSS) to enable the LSS to generate localization signals in synchronization with the sensing windows.

Abstract: A swallowable in-vivo device contains a movement detection unit that includes a movement sensing unit, a frequency analyzing unit (FAU) and a time analyzing unit (TAU). The movement sensing unit senses movements of the in-vivo device relative to a non-stationary three-dimensional reference frame, and outputs a movement signal. The frequency analyzing unit may analyze the movement signal spectrally to detect a potential command-invoking movement, and the time analyzing unit may analyze the potential CIM temporally, possibly in conjunction with a series of other movement events, to determine whether the potential CIM is a genuine CIM. If the potential CIM is determined to be a genuine CIM, the in-vivo device may execute a predetermined command associated with the CIM. Otherwise, the in-vivo device may refrain from executing a CIM-related command. A PCB including the movement detection unit and a processor for processing their output is provided for the vivo sensing device.

Abstract: A control circuit for controlling a state of a switching circuit may include a first unit to sense and interpret a wireless signal or physical parameter as an “on” signal to transition the switching circuit to the “on” state, or as an “off” signal to transition the switching circuit to the “off” state, and to transfer a first digital signal or logic value and/or a second digital signal or logic value, which may respectively or combinatorially represent the “on” signal or the “off” signal, to a second unit via a first output and/or a second output of the first unit, respectively. The second unit may force a control input of the switching circuit to a logic value which is a function of the first digital signal or value and/or second digital signal or value and congruent with the state to which the switching circuit is to be transitioned.

Abstract: An electromagnetic localization signal may be sensed by an electromagnetic field sensor in an in-vivo device with an electromagnetic field interference that is superimposed on the electromagnetic localization signal. The electromagnetic field interference may be filtered by outputting, by the electromagnetic field sensor, an alternating signal that represents, or in response to, the electromagnetic localization signal; sampling a first (e.g., positive) portion of the alternating signal during a first sampling window or period to obtain a first set of samples, sampling a second (e.g., negative) portion of the alternating signal during a second sampling window or period to obtain a second set of samples; and calculating a number, NR, from the first and second sets of samples, that approximately represents a substantially interference free electromagnetic localization signal.

Abstract: An in-vivo device may allocate a sensing window in a work cycle for sensing and processing localization signals to determine the location/orientation of the in-vivo device. The in-vivo device may use a timing unit to schedule transmission of data frames and the sensing of the localization signals relative to a reference time embedded in work cycles. The timing unit may produce a clock signal based on which the in-vivo device may measure time specifics, which define the sensing window, relative to the reference time. A receiver may use data embedded in data frames to restore the clock signal and the reference time, and, from them, and using identical or similar time specifics, generate a synchronization signal for a localization signals source (LSS) to enable the LSS to generate localization signals in synchronization with the sensing windows.