Abstract: A biochip includes a perfusion layer between a media layer and a first membrane. The perfusion layer has a perfusion channel to provide a liquid to cells in a well layer, with the cells attached to a second membrane below the well layer. A stimulation layer below the second membrane includes a stimulation air channel having a stimulation channel inlet and a stimulation channel outlet. The biochip may be used to detect volatile organic compounds in air.

Abstract: A universal odor code system encodes an olfactory stimulus into olfactory receptor space as quantitative measures of responses by olfactory receptors, producing an odor code profile. A mapping function maps an odor code profile into a formula of elements that approximates or recreates an odor code profile of a target olfactory stimulus.

Abstract: A system comprising (1) a microelectrode array (MEA) component comprising an integrated multiplexed (MUX) logic circuit; and (2) a microprocessor, e.g., MOSFET, such as a CMOS, wherein the MEA is in electrical communication with the microprocessor such that signals produced by the microelectrodes are transmitted to the processor through the MUX. The use of a MUX reduces the number of outputs used to communicate signals from multiple microelectrodes to the microprocessor. The two components are removably engageable with each other such that after one or more uses, the engaged MEA can be removed and replaced with a new MEA, without the necessity of disposing the microprocessor.

Abstract: Cells in wells of a microplate may be directly stimulated with gases, vapors or volatile organic compounds in a scalable way while remaining compliant with existing standards. The microplate may be used in designing of GPCR's (G protein-coupled receptors), in biotechnology and synthetic biology, drug development, oxygenation studies, food flavor design and others. An assay system for operating with gas stimulation of cells includes an optical sensor positioned to detect light in the wells.

Abstract: A biochip for detection of volatile organic compounds in air includes one or more wells for holding living cells. A capillary connecting each well to a liquid source may be used. The liquid source may be an on-chip reservoir or a system liquid supply. An air flow channel is separated from each well by a membrane. At least a portion of the biochip is transparent to allow optical detection of cell fluorescence. The biochip may be made of multiple flat transparent layers attached together. A system for detecting volatile organic compounds in air has an optical system adapted to detect fluorescence of genetically modified living cells expressing an odorant receptor capable of binding to the volatile organic compound and a calcium sensitive fluorescent reporter that fluoresces in response to binding of the volatile organic compound to the odorant receptor.

Abstract: Provided herein is a system comprising (1) a microelectrode array (MEA) component comprising an integrated multiplexed (MUX) logic circuit; and (2) a microprocessor, e.g., MOSFET, such as a CMOS, wherein the MEA is in electrical communication with the microprocessor such that signals produced by the microelectrodes are transmitted to the processor through the MUX. The use of a MUX reduces the number of outputs used to communicate signals from multiple microelectrodes to the microprocessor. The two components are removably engageable with each other such that after one or more uses, the engaged MEA can be removed and replaced with a new MEA, without the necessity of disposing the microprocessor.

Abstract: Provided herein is a cell culture module for culturing one or more cells in an array of chambers formed therein the cell culture module. In some cases, the cell culture module may further comprise one or more sensors. In some cases, the cell culture module may further comprise one or more electrodes.

Abstract: Provided herein is a system comprising (1) a microelectrode array (MEA) component comprising an integrated multiplexed (MUX) logic circuit; and (2) a microprocessor, e.g., MOSFET, such as a CMOS, wherein the MEA is in electrical communication with the microprocessor such that signals produced by the microelectrodes are transmitted to the processor through the MUX. The use of a MUX reduces the number of outputs used to communicate signals from multiple microelectrodes to the microprocessor. The two components are removably engageable with each other such that after one or more uses, the engaged MEA can be removed and replaced with a new MEA, without the necessity of disposing the microprocessor.

Abstract: Neuronal gate devices that function as a biological equivalent of AND, OR, and NOT digital logic gates are described. The neuronal gate devices comprise microstructures that support the patterned growth of two or more populations of neurons that are synaptically coupled. Also described are reconfigurable biological computers that comprise two or more coupled neuronal gates and may be trained to process a set of one or more input signals and generate a corresponding set of one or more output signals.

Abstract: Disclosed here are methods and systems for assessing an emotional response of a subject or a group to a sensory stimulus. The methods employ models that infer emotional response based on individual traits of subjects or groups.

Abstract: A device housing that is designed to maximize the dwell time for volatile compounds drawn into a cell-based compound detection device is described. The modular functional components of the cell-based detection devices are also described.

Abstract: Provided herein is a cell culture module for culturing one or more cells in an array of chambers formed therein the cell culture module. In some cases, the cell culture module may further comprise one or more sensors. In some cases, the cell culture module may further comprise one or more electrodes.