Patents by Inventor Palani Kumaresan
Palani Kumaresan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8841116Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.Type: GrantFiled: October 25, 2007Date of Patent: September 23, 2014Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
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Patent number: 8454906Abstract: Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.Type: GrantFiled: July 24, 2008Date of Patent: June 4, 2013Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Palani Kumaresan, Chaoyang Yang, Robert G. Blazej
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Patent number: 8420318Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.Type: GrantFiled: February 13, 2012Date of Patent: April 16, 2013Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Robert Blazej, Chung Liu, Palani Kumaresan, Stephanie H. I. Yeung
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Publication number: 20120142010Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.Type: ApplicationFiled: February 13, 2012Publication date: June 7, 2012Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Richard A. MATHIES, Robert BLAZEJ, Chung LIU, Palani KUMARESAN, Stephanie H.I. YEUNG
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Publication number: 20110020920Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.Type: ApplicationFiled: July 27, 2010Publication date: January 27, 2011Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Richard A. MATHIES, Robert BLAZEJ, Chung LIU, Palani KUMARESAN, Stephanie H. I. YEUNG
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Publication number: 20100285975Abstract: Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.Type: ApplicationFiled: July 24, 2008Publication date: November 11, 2010Applicant: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Palani Kumaresan, Chaoyang Yang, Robert G. Blazej
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Patent number: 7799553Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.Type: GrantFiled: May 25, 2005Date of Patent: September 21, 2010Assignee: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Robert Blazej, Chung Liu, Palani Kumaresan, Stephanie H. I. Yeung
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Publication number: 20090035770Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.Type: ApplicationFiled: October 25, 2007Publication date: February 5, 2009Applicant: The Regents of the University of CaliforniaInventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
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Publication number: 20050287572Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.Type: ApplicationFiled: May 25, 2005Publication date: December 29, 2005Applicant: The Regents of the University of CaliforniaInventors: Richard Mathies, Robert Blazej, Chung Liu, Palani Kumaresan, Stephanie Yeung