Patents by Inventor Pamela S. Olson

Pamela S. Olson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
    Patent number: 5914254
    Abstract: The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.
    Type: Grant
    Filed: May 12, 1997
    Date of Patent: June 22, 1999
    Assignee: Celtrix Pharmaceuticals, Inc.
    Inventors: Desmond Mascarenhas, Yang Zhang, Pamela S. Olson, David R. Olsen, Pedro A. Cohen, Kimyen B. Nguyen
  • Chromosomal expression of heterologous genes in bacterial cells
    Patent number: 5861273
    Abstract: The present invention provides compositions and methods for producing a heterologous protein of interest by inserting a copy of a gene encoding the heterologous protein of interest into the chromosome of a host cell, such as E. coli. A chromosomal transfer DNA (a circular, non-self-replicating DNA) is used to integrate the gene encoding the heterologous protein of interest into the host cell chromosome. The chromosomal transfer DNA comprises at least one selectable marker and may optionally include repeated DNA sequences flanking the selectable marker, facilitating chromosomal amplification of the integrated DNA. The gene encoding the protein of interest may be expressed after integration into the chromosome of the host cell; selection for chromosomal amplification may be performed prior to expression of the gene.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 19, 1999
    Assignee: Celtrix Phamraceuticals, Inc.
    Inventors: Pamela S. Olson, Desmond Mascarenhas
  • Polypeptide fusions to polypeptides of the beta-trefoil fold structural family
    Patent number: 5830706
    Abstract: The invention is directed to the use of IL-1-like fusion polypeptides to increase the solubility and activity of recombinant polypeptides. The invention includes a nucleic acid encoding a fusion polypeptide comprising an interleukin-1-like polypeptide and a polypeptide of interest and the method of using such a nucleic acid to produce recombinant fusion polypeptides.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: November 3, 1998
    Assignee: Celtrix Pharmaceuticals, Inc.
    Inventors: Desmond Mascarenhas, Yang Zhang, Pamela S. Olson, David R. Olsen, Pedro A. Carrillo
  • Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
    Patent number: 5629172
    Abstract: The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.
    Type: Grant
    Filed: August 2, 1994
    Date of Patent: May 13, 1997
    Assignee: Celtrix Pharmaceuticals, Inc.
    Inventors: Desmond Mascarenhas, Yang Zhang, Pamela S. Olson, David R. Olsen, Pedro A. Cohen
  • Fusion polypeptides and proteins
    Patent number: 5563046
    Abstract: The invention is directed to the use of IL-1-like fusion polypeptides to increase the solubility and activity of recombinant polypeptides. The invention includes a nucleic acid encoding a fusion polypeptide comprising an interleukin-1-like polypeptide and a polypeptide of interest and the method of using such a nucleic acid to produce recombinant fusion polypeptides.
    Type: Grant
    Filed: August 2, 1993
    Date of Patent: October 8, 1996
    Assignee: Celtrix Pharmaceuticals, Inc.
    Inventors: Desmond Mascarenhas, Yang Zhang, Pamela S. Olson, David R. Olsen, Pedro A. Carrillo
  • Chromosomal expression of non-bacterial genes in bacterial cells
    Patent number: 5470727
    Abstract: The present invention provides compositions and methods for inserting a copy of a heterologous gene into the chromosome of a host cell such as E. coli through the use of a chromosomal transfer DNA, a circular, non-self-replicating DNA carrying a site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
    Type: Grant
    Filed: December 21, 1993
    Date of Patent: November 28, 1995
    Assignee: Celtrix Pharmaceuticals, Inc.
    Inventors: Desmond Mascarenhas, Pamela S. Olson