Abstract: Disclosed are systems and methods for simultaneous detection of DNA and RNA genetic alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient without physically separating RNA from DNA. The systems and methods are similarly applicable to the simultaneous detection of DNA and RNA genetic alterations in solid tissues comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof. The present method utilized a barcoding method for analysis. The streamlined methods improve the simplicity, quantification accuracy and detection sensitivity and specificity of non-invasive detections of biomarkers.

Abstract: Provided herein are devices and methods for collection of a biological sample. The device may comprise a cap, a pierceable container comprising a reagent for processing or preserving a biological sample, and a container that comprises a piercer. Upon engagement of the cap with an opening of the container, the piercer may be able to pierce a pierceable container, thereby releasing the reagent of the pierceable container into the container. The methods may comprise use of the device to collect a biological sample. The methods may comprise assaying analytes of the biological sample.

Abstract: Provided herein are methods and systems monitoring of cancer using minimal residual disease analysis. The methods may comprise assaying multiple nucleic acids to detect a set of biomarkers from samples. The methods may comprise sequencing of nucleic acids. The method may comprise the generation of a probe panel. The methods may comprise processing the set of biomarkers to determine the presence of a cancer or cancer parameters. The processing may be performed by an algorithm.

Abstract: Disclosed are systems and methods for detecting genetic alterations comprising androgen receptor gene splice variants (AR-Vs), mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient. The systems and methods are similarly applicable to the detection of gene alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof of other genes of interest. The streamlined methods improve the consistency and simplicity of non-invasive detections of biomarkers.

Abstract: Disclosed are systems and methods for detecting genetic alterations comprising androgen receptor gene splice variants (AR-Vs), mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient. The systems and methods are similarly applicable to the detection of gene alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof of other genes of interest. The streamlined methods improve the consistency and simplicity of non-invasive detections of biomarkers.

Abstract: An in-situ test device and method for surrounding rock strength of bolt supported roadway is provided. The test device includes a fixing mechanism, a loading mechanism, a measuring mechanism and a control system. The loading mechanism includes a hydraulic pump and a plunger pump, the hydraulic pump drives the plunger pump to work and controls the lifting speed of the loading cylinder; the measuring mechanism includes an infrared ranging unit and a wireless pressure monitoring unit; the control system controls the work of the loading mechanism and processes the monitoring data. The test device is directly installed in the roadway and fixed with the bolt. The device is loaded after leveling, the device is disassembled after the monitoring data are obtained, and the in-situ test for the surrounding rock strength of the bolt supported roadway is completed. The steps are simple and adaptable.

Abstract: An in-situ test device and method for surrounding rock strength of bolt supported roadway is provided. The test device includes a fixing mechanism, a loading mechanism, a measuring mechanism and a control system. The loading mechanism includes a hydraulic pump and a plunger pump, the hydraulic pump drives the plunger pump to work and controls the lifting speed of the loading cylinder; the measuring mechanism includes an infrared ranging unit and a wireless pressure monitoring unit; the control system controls the work of the loading mechanism and processes the monitoring data. The test device is directly installed in the roadway and fixed with the bolt. The device is loaded after leveling, the device is disassembled after the monitoring data are obtained, and the in-situ test for the surrounding rock strength of the bolt supported roadway is completed. The steps are simple and adaptable.

Abstract: The cleaning device includes a water injection member and a mopping cloth cleaning member. The water injection member is configured to wet the mopping cloth of a cleaning robot. The mopping cloth cleaning member which reciprocates up and down to flap the mopping cloth of the cleaning robot, in order to clean the mopping cloth of the cleaning robot.

Abstract: Disclosed are systems and methods for simultaneous detection of DNA and RNA genetic alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient without physically separating RNA from DNA. The systems and methods are similarly applicable to the simultaneous detection of DNA and RNA genetic alterations in solid tissues comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof. The present method utilized a barcoding method for analysis. The streamlined methods improve the simplicity, quantification accuracy and detection sensitivity and specificity of non-invasive detections of biomarkers.

Abstract: Disclosed are systems and methods for simultaneous detection of DNA and RNA genetic alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient without physically separating RNA from DNA. The systems and methods are similarly applicable to the simultaneous detection of DNA and RNA genetic alterations in solid tissues comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof. The present method utilized a barcoding method for analysis. The streamlined methods improve the simplicity, quantification accuracy and detection sensitivity and specificity of non-invasive detections of biomarkers.

Abstract: Disclosed are systems and methods for diagnosing a disease status or progression of a disease comprising detection and correlation of methylation and expression level changes of cancer markers including PD-L1 in a biofluid sample from the patient. The systems and methods are similarly applicable to the detection and correlation of methylation and expression level changes in solid tissues. The present method utilized a barcoding method for analysis. The systems and methods can be used for predicting immunotherapy resistant cancer and monitoring therapy response.

Abstract: Disclosed are systems and methods for simultaneous detection of DNA and RNA genetic alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient without physically separating RNA from DNA. The systems and methods are similarly applicable to the simultaneous detection of DNA and RNA genetic alterations in solid tissues comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof. The present method utilized a barcoding method for analysis. The streamlined methods improve the simplicity, quantification accuracy and detection sensitivity and specificity of non-invasive detections of biomarkers.

Abstract: Disclosed are systems and methods for detecting genetic alterations comprising androgen receptor gene splice variants (AR-Vs), mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient. The systems and methods are similarly applicable to the detection of gene alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof of other genes of interest. The streamlined methods improve the consistency and simplicity of non-invasive detections of biomarkers.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.