Abstract: The present invention relates to covalently cross-linked glycosylated mucin nanoparticles and the use thereof for the delivery and release of active ingredients, markers and/or biomolecules. The subject matter of the invention also relates to covalently cross-linked glycosylated mucin nanoparticles comprising at least one compound selected from an active ingredient, a marker and a biomolecule. The invention further relates to a method for preparing covalently cross-linked glycosylated mucin nanoparticles, optionally comprising at least one compound selected from an active ingredient, a marker and a biomolecule.

Abstract: A millifluidic device for cultures of biological agents comprising: a main body (11) comprising at least a first hole (40) closed at the bottom; a separator (35); a membrane (36) fixed to said separator (35); a plug (15) closing said first hole (40); said separator (35) designed to be placed in said first hole (40); said separator (35) being extractable from said first hole (40); said membrane (36) divides said first hole (40 ) into an upper half-chamber and a lower half-chamber; a pair of tubes (25) to perfuse said lower half-chamber; a pair of tubes (26) to perfuse said upper half-chamber; a first slide (22) placed centrally on said plug (15); a second slide (42) placed centrally on said first hole (40); a cylindrical body (41) rises from said first hole (40) and said second slide (42) is placed on the top of said cylindrical body (41); said cylindrical body (41) has a second hole (43), coaxial to said cylindrical body (41).

Abstract: A three-dimensional substrate with structural and compositional gradient for microbial cultures and cocultures is provided. The three-dimensional substrate includes a diffusion system having a first apical compartment placed above a second basolateral compartment, the first and second compartments being separated by a semipermeable membrane, a base solution comprising polysaccharides, proteins and salts or a preformed hydrogel comprising polysaccharides, proteins and salts, and a cross-linking medium comprising salts, culture media and distilled water. A method for preparing a three-dimensional substrate with structural and compositional gradient for microbial cultures and cocultures is also provided.

Abstract: A method for obtaining a composite material including an aqueous solution of pectin and a suspension/solution of calcium phosphate mixed together, wherein said solution of pectin cross-links with a portion of the calcium obtained from the solution of calcium phosphate and wherein a portion of the calcium phosphate in suspension remains as inorganic phase and composite materials obtained by this method.

Abstract: A method for obtaining a composite material including an aqueous solution of pectin and a suspension/solution of calcium phosphate mixed together, wherein said solution of pectin cross-links with a portion of the calcium obtained from the solution of calcium phosphate and wherein a portion of the calcium phosphate in suspension remains as inorganic phase and composite materials obtained by this method.