Abstract: The present disclosure relates to a process for the preparation of core-shell particles by the coacervation method encapsulating contrast agents for multimodal imaging. The process consists in: a. Providing a water in oil emulsion of a biocompatible polyelectrolyte polymer. b. Providing an aqueous solution of a biocompatible polyelectrolyte polymer having opposite charges of the polyelectrolyte of step a). c. Adding a crosslinking agent to the primary emulsion and the secondary solution. d. Adding at least a tracer independently to the primary emulsion or the secondary solution or emulsion. e. Adding the secondary aqueous solution to the primary emulsions and occurring of the complex coacervation leading to the separation of the coacervate particles. f. Optionally absorb a further tracer into the nanoparticles The disclosure also relates to the coacervates obtained by the above described process and their use as probe for multimodal imaging in the diagnostic field.

Abstract: The present disclosure relates to a process for the preparation of core-shell particles by the coacervation method encapsulating contrast agents for multimodal imaging. The process consists in: a. Providing a water in oil emulsion of a biocompatible polyelectrolyte polymer. b. Providing an aqueous solution of a biocompatible polyelectrolyte polymer having opposite charges of the polyelectrolyte of step a). c. Adding a crosslinking agent to the primary emulsion and the secondary solution. d. Adding at least a tracer independently to the primary emulsion or the secondary solution or emulsion. e. Adding the secondary aqueous solution to the primary emulsions and occurring of the complex coacervation leading to the separation of the coacervate particles. f. Optionally absorb a further tracer into the nanoparticles The disclosure also relates to the coacervates obtained by the above described process and their use as probe for multimodal imaging in the diagnostic field.

Abstract: There is disclosed a kit for detecting a single strand target nucleotide sequence comprising: at least one first nucleic acid probe from 10 to 14 bases, to the 5? end of which at least one fluorophore is bound; at least one second nucleic acid probe from 35 to 50 bases, comprising, from the 5? to the 3? end: a first segment having a nucleotide sequence complementary to the first nucleic acid probe, at least one quencher, and a second segment having a nucleotide sequence complementary to at least part of the target nucleotide sequence, wherein the following relation is met: |?G hybr.target3?probe2|>|?G hybr.probe1?probe2|.

Abstract: There is disclosed a kit for detecting a single strand target nucleotide sequence comprising: at least one first nucleic acid probe from 10 to 14 bases, to the 5? end of which at least one fluorophore is bound; at least one second nucleic acid probe from 35 to 50 bases, comprising, from the 5? to the 3? end: a first segment having a nucleotide sequence complementary to the first nucleic acid probe, at least one quencher, and a second segment having a nucleotide sequence complementary to at least part of the target nucleotide sequence, wherein the following relation is met: |?G hybr.target3?probe2|>|?G hybr.probe1?probe2|.

Abstract: The present disclosure relates to mold components and imprint lithography techniques applied on the basis of organic mold materials in order to form polymer microstructure elements. It has been recognized that adapting surface characteristics of at least one mold component may significantly enhance performance of the lithography process, in particular with respect to suppressing residual polymer material, which in conventional strategies may have to be removed on the basis of an additional etch process.

Abstract: Cells in a matrix or in the matrix in a vessel are grown to form a multicellular aggregate. Pressure is exerted on the growing cells by the matrix or the matrix together with the vessel due to growing cellular mass displacing the matrix. A value representing pressure exerted on the cells is calculated and the pressure is modulated to control growth of the multicellular aggregate, or to produce a multicellular aggregate of a pre-selected size or a pre-selected size and shape. Matrices include agarose, alginate and collagen gels, and the pressure exerted on the cells can be non-isotropic. The cells may be tumor cells, or liver, pancreatic, brain, skin, bone or muscle cells, and cell growth can be in vitro or in vivo. When collagen forms the matrix, the matrix may contain glycosaminoglycan.

Abstract: The invention provides a method of enhancing the amount of a pharmaceutical composition delivered to a target tissue site in a mammal, by creating a transient differential between tile hydrostatic pressure in the target site and a region near the target tissue site whereby the composition is transported toward the site. An apparatus for performing the method is provided. In one form that apparatus includes a pharmaceutical reservoir, pump, and an agent reservoir and pump.