Abstract: Novel nucleic acid sequences encoding hemipteran L-glutamate decarboxylases, and the amino acid sequence of such protein are disclosed. Methods of making and using the same are disclosed.

Abstract: Novel nucleic acid sequences encoding hemipteran myosin light chain kinases are disclosed. Isolated hemipteran myosin light chain kinases are also disclosed.

Abstract: The present invention discloses a human embryo kidney cell or cell line transfected with a GABA-gated chloride channel derived from an insect of the order of Lepidoptera, Diptera, Coleoptera, Homoptera, Acarina, Thysanaptera, Heteroptera, Hymenoptera or Isoptera and its use in a one-pot, high-throughput method for the measurement of the change in membrane potential in a cell or cell line. The method is particularly effective in measuring changes in membrane potential resulting from a compound acting on a GABA-gated chloride channel derived from a tobacco budworm.

Abstract: The present invention provides a recombinant plasmid adapted for transformation of a host microorganism, the plasmid comprising a plasmid vector into which a polydeoxyribonucleotide segment that codes for ammonia monooxygenase and hydroxylamine oxidoreductase has been inserted, wherein the resulting transformant exhibits at least 50% of the overall level of ammonia monooxygenase and hydroxylamine oxidoreductase activity of the donor. Also provided are an isolated polydeoxyribonucleotide segment that encodes ammonia monooxygenase and hydroxylamine oxidoreductase, and a transformant containing the ammonia monooxygenase and hydroxylamine oxidoreductase gene segments.

Abstract: An economical method of converting mannose to fructose uses a mannose isomerase from Pseudomonas cepacia immobilized on an alumina containing polyethyleneimine crosslinked with an excess of glutaraldehyde. The method utilizes mannose-containing aqueous solutions as the feedstock, and affords solutions in which at least 55% of the mannose has been converted to fructose. Because of the relatively higher levels of fructose than can be obtained by isomerizing glucose to fructose using glucose isomerase, substantial savings in separation of high fructose-containing products can be achieved. The process described represents the first economical mannose isomerase process.

Abstract: Two Bacillus species and one Pseudomonas species isolated from soil samples showed good cyclodextrin glycosyltransferase activity, even when grown in simple media. The microorganisms are not pH sensitive and grow well in media containing only starch, a nitrogen source, and minerals. All elaborate the desired enzyme within 4 hours of culturing with growth being complete within about 24 hours.

Abstract: Corn kernel hulls are found to be an essentially lignin-free material which is readily hydrolyzed to a mixture principally of three monosaccharides: D-glucose, D-xylose, and L-arabinose. Several discrete processing methods employing a combination of acid and enzymatic hydrolysis afford product streams rich either in glucose or in a mixture of the two pentoses, D-xylose and L-arabinose.