Abstract: The present disclosure describes biocatalytic processes for producing a product, comprising providing an aqueous stream (AS) comprising at least one fermentable substrate and a gaseous stream (GS) comprising at least one of CO2/H2, H2, methane, and/or CO to a fermentation zone, wherein the GS and AS stream are optionally contacted and/or mixed; the fermentation zone comprising at least one organism capable of metabolizing an AS substrate and a GS substrate, wherein the fermentation operates at conditions to mixotrophically metabolize at least one gaseous substrate in the GS and at least one substrate in the AS, producing the product.

Abstract: Methods, systems and compositions for producing at least one light-boiling, volatile, organic product using at least a portion of one or more carbon containing substances from a non-biosynthetic process in a biosynthetic process are provided. These methods, systems and compositions are useful in reducing waste treatment load of carbon containing chemical process waste streams.

Abstract: The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

Abstract: Methods for biosynthesising hydrocarbons from a gaseous substrate in non-naturally occurring acetogens as well as non-naturally occurring acetogens for production of hydrocarbons are provided.

Abstract: Nutritive compositions containing an organism having attenuated or no poly(3-hydroxybutyrate) production are provided. These nutritive compositions are useful as feed, such as animal feed and feed additives for ruminants, non-ruminants and aquaculture, etc. Materials and methods for production of these organisms are provided.

Abstract: This disclosure relates to strategies for in vivo production of certain carbon-based products, for example, aminated aliphatic compounds having a carbon chain length of C5-C19.

Abstract: This document describes a process for the high purity and high concentration recovery of monovalent products via continuous ion exchange from aqueous solution for further down-stream purification.

Abstract: Provided herein are novel, synthetic polypeptides having, for example, acyl-acyl carrier protein (ACP) thioesterase (TE) activity, including polypeptides that convert pimeloyl-ACP to pimelic acid. In some aspects, the synthetic polypeptides have advantageous enzymatic activity and/or improved substrate specificity relative to a wild type acyl-ACP TE.

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract: The present disclosure generally relates to methods for the recovery of amines from aqueous mixtures. In particular, the disclosure relates to methods for separating amines from amine-containing aqueous mixtures by adjusting the pH of the aqueous mixture relative to the highest pKa value for the amines.

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract: The present disclosure describes biocatalytic processes for producing a product, comprising providing an aqueous stream (AS) comprising at least one fermentable substrate and a gaseous stream (GS) comprising at least one of CO2/H2, H2, methane, and/or CO to a fermentation zone, wherein the GS and AS stream, are optionally contacted and/or mixed; the fermentation zone comprising at least one organism capable of metabolizing an AS substrate and a GS substrate, wherein the fermentation operates at conditions to mixotrophically metabolize at least one gaseous substrate in the GS and at least one substrate in the AS, producing the product.

Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.

Abstract: Methods, systems and compositions for producing at least one light-boiling, volatile, organic product using at least a portion of one or more carbon containing substances from a non-biosynthetic process in a biosynthetic process are provided. These methods, systems and compositions are useful in reducing waste treatment load of carbon containing chemical process waste streams.

Abstract: Provided herein are novel, synthetic polypeptides having, for example, acyl-acyl carrier protein (ACP) thioesterase (TE) activity, including polypeptides that convert pimeloyl-ACP to pimelic acid. In some aspects, the synthetic polypeptides have advantageous enzymatic activity and/or improved substrate specificity relative to a wild type acyl-ACP TE.

Abstract: This disclosure relates to strategies for in vivo production of certain carbon-based products, for example, aminated aliphatic compounds having a carbon chain length of C5-C19.

Abstract: Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

Abstract: The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

Abstract: Disclosed are materials and methods for managing aerobic biosynthesis. The materials include a fermenter system comprising a fermenter, a microorganism provided to the fermenter, and at least two control loops. The methods are directed to measuring and controlling different oxygen concentrations within the fermenter.

Abstract: Nutritive compositions containing an organism having attenuated or no poly(3-hydroxybutyrate) production are provided. These nutritive compositions are useful as feed, such as animal feed and feed additives for ruminants, non-ruminants and aquaculture, etc. Materials and methods for production of these organisms are provided.