Patents by Inventor Paulus Petrus DE WAAL
Paulus Petrus DE WAAL has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117299Abstract: The invention relates to the field of dairy technology. The invention specifically relates to a Streptococcus thermophilus starter strain and composition comprising such strain, to methods to produce a fermented milk product using such strain or composition and to dairy products produced using such strain or composition.Type: ApplicationFiled: October 11, 2023Publication date: April 11, 2024Inventors: Lourdes Mariela SERRANO DAVALOS, Paul KLAASSEN, Ali MAY, Sofia DASHKO, Claire Emile PRICE, Paulus Petrus DE WAAL
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Patent number: 11624057Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: March 17, 2021Date of Patent: April 11, 2023Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Publication number: 20220325253Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 17, 2021Publication date: October 13, 2022Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Patent number: 11384368Abstract: The invention relates to a recombinant yeast comprising a recombinant yeast comprising a nucleotide sequence coding for a glycerol dehydrogenase, a nucleotide sequence coding for a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39); a nucleotide sequence coding for a phosphoribulokinasey (EC 2.7.1.19); a nucleotide sequence allowing the expression of a glucoamylase (EC 3.2.1.20 or 3.2.1.3); and optionally a nucleotide sequence coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: September 25, 2018Date of Patent: July 12, 2022Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz
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Publication number: 20220204947Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 17, 2021Publication date: June 30, 2022Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Publication number: 20220205056Abstract: The present invention relates to a kit suitable for detection and quantification of phage DNA from a lactic acid bacteria infecting phage in a dairy sample. The invention provides a quantitative polymerase chain reaction (qPCR) kit for detection and quantification of phage DNA to from a lactic acid bacteria infecting phage in a dairy sample, said kit comprising a first primer pair and wherein said first primer pair has a robustness of a delta Cq lower than 1.0 cycle when tested in a temperature range of 55.0-70.0, preferably 55.0-68.0, more preferably 58.6-65.6 degrees Celsius and wherein said first primer pair is directed to a lactic acid bacteria infecting phage which is a lactococcal phage from the subgroup 9362, c2 or P335 or a streptococcal phage from the subgroup pac.Type: ApplicationFiled: April 15, 2020Publication date: June 30, 2022Inventors: Paulus Petrus DE WAAL, Laurens Leendert HANEMAAIJER
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Patent number: 11319543Abstract: The present invention relates to a yeast cell that is genetically modified comprising: a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast; b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.Type: GrantFiled: May 18, 2016Date of Patent: May 3, 2022Assignee: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Jozef Petrus Johannes Schmitz, Paulus Petrus De Waal, Arjen Bouma
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Patent number: 11274310Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: September 25, 2018Date of Patent: March 15, 2022Assignee: DSM IP ASSETS B.V.Inventors: Hans Marinus Charles Johannes De Bruijn, Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz
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Patent number: 11203741Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: June 11, 2021Date of Patent: December 21, 2021Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Patent number: 11186850Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: GrantFiled: April 24, 2020Date of Patent: November 30, 2021Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Publication number: 20210317420Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: June 11, 2021Publication date: October 14, 2021Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Publication number: 20210310013Abstract: The present invention relates to a yeast cell that is genetically modified comprising: a) a disruption of one or more aldehyde dehydrogenase (E.C: 1.2.1.4) native to the yeast; b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.Type: ApplicationFiled: June 14, 2021Publication date: October 7, 2021Inventors: PAUL KLAASSEN, JOZEF PETRUS JOHANNES SCHMITZ, PAULUS PETRUS DE WAAL, ARJEN BOUMA
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Patent number: 10982195Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: December 18, 2017Date of Patent: April 20, 2021Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Publication number: 20200407733Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: September 25, 2018Publication date: December 31, 2020Inventors: Hans Marinus Charles Johannes DE BRUIJN, Paulus Petrus DE WAAL, Ingrid Maria VUGT-VAN LUTZ
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Publication number: 20200392513Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a genome editing system.Type: ApplicationFiled: November 20, 2018Publication date: December 17, 2020Inventors: Rene VERWAAL, Paulus Petrus DE WAAL, Francine Maruschka Johanna DE LEEUW-VAN LOON
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Publication number: 20200283805Abstract: The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less.Type: ApplicationFiled: September 25, 2018Publication date: September 10, 2020Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Publication number: 20200255871Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: ApplicationFiled: April 24, 2020Publication date: August 13, 2020Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Paulus Petrus DE WAAL, Niels LAURET, Maarten Dirk VERHOEVEN, Jozef Petrus Johannes SCHMITZ
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Publication number: 20200239914Abstract: The invention relates to a recombinant yeast comprising a recombinant yeast comprising a nucleotide sequence coding for a glycerol dehydrogenase, a nucleotide sequence coding for a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39); a nucleotide sequence coding for a phosphoribulokinasey (EC 2.7.1.19); a nucleotide sequence allowing the expression of a glucoamylase (EC 3.2.1.20 or 3.2.1.3); and optionally a nucleotide sequence coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: September 25, 2018Publication date: July 30, 2020Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ
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Patent number: 10689670Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: GrantFiled: June 13, 2017Date of Patent: June 23, 2020Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Publication number: 20200024619Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 20, 2018Publication date: January 23, 2020Inventors: Paulus Petrus DE WAAL, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN