Abstract: The present disclosure relates in general, to quality control compositions and whole organism control materials for use in assessing the functionality of Nucleic Acid Tests (NAT) and to related methods, uses and kits. In some aspects the NAT is used to detect the presence of an organism and a target nucleic acid sequence in a test article, the quality control composition comprising: (i) a first protected nucleic acid sequence comprising a first nucleic acid sequence the presence of which is characteristic of the presence of the organism in the test article said first nucleic acid sequence being tested in the NAT; and (ii) a second protected nucleic acid sequence comprising a second nucleic acid sequence the presence of which is characteristic of the presence of the target nucleic acid sequence in the test article said second nucleic acid sequence being tested for in the NAT.

Abstract: Water soluble, polyethylenimine polymers, conjugated to one or more active moiety are taught. Also taught are methods of making same, kits comprising same, and use of same in immunoassay systems.

Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.

Abstract: Water soluble, polyethylenimine polymers, conjugated to one or more active moiety are taught. Also taught are methods of making same, kits comprising same, and use of same in immunoassay systems.

Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.