Abstract: A culture medium composition for expanding hematopoietic stem cells (HSCs) and maintaining self-renewal capacity and differentiation potential of HSCs, a cell population and an application thereof. The culture medium composition comprises a hematopoietic stem cell medium and a small molecule inhibitor of a PDGFR target. The inhibitor of PDGFR can significantly expand HSCs during in-vitro culture, maintaining a high proportion of HSCs with self-renewal capacity, achieving in-vitro expansion of HSCs while maintaining a relatively high proportion of cells with sternness.

Abstract: Provided is the use of a compound for improving the transplantation efficiency of human hematopoietic stem cells, wherein the compound is selected from one of or two or more of a tubulin polymerization inhibitor, LND-212854, AZD0364, SCH772984, Pimasertib, Trametinib, IWR-1-endo, TTNPB, JNK-inhibitor IX and CHIR99021. The compound provided can improve the expression of the hematopoietic stem cell surface protein CD184 by means of in vitro short-time treatment, without affecting the phenotype, survival rate and various characteristics of the cell. By means of the binding of the chemokine receptor CD184 expressed by HSC to the chemokine ligand SDF1 in the bone marrow microenvironment, the migration ability of the HSC and the homing ability thereof in the bone marrow are improved, and the transplantation efficiency and the therapeutic effect of the HSC are enhanced.

Abstract: Provided are a cell preparation, a use of a protein in characterizing hematopoietic stem cells, and a method for determining hematopoietic stem cells. The cell preparation is CD34+, CD90+, CD45RA? sorted and obtained from among cells to be sorted, and hematopoietic stem cells that are positive for the following proteins, the protein being selected from one or more among CD48, CD66a, CD66c, CD66d, CD66e, and CD200. By using the foregoing protein(s) to characterize hematopoietic stem cells, not only can target cells be more accurately calibrated and evaluated in vitro, but the ability of a patient to maintain long-term reconstruction of the hematopoietic system after receiving a hematopoietic stem cell transplant can also be improved, achieving a better treatment effect.

Abstract: Provided is a method for editing a target RNA at a target residue position of a host cell. The method comprises introducing ADAR-recruiting RNA (arRNA) or a construct encoding the arRNA into a host cell, wherein the arRNA comprises a complementary RNA sequence that hybridizes to a target RNA; the target residue is located in a three-base motif comprising a 5? nearest-neighbor residue (upstream residue) of the target residue in the target RNA, the target residue, and a 3? nearest-neighbor residue (downstream residue) of the target residue in the target RNA, wherein the three-base motif is not UAG, and the complementary RNA sequence comprises a mismatch directly opposite to the upstream residue or the downstream residue in the target RNA. Further provided are arRNA for the method, an RNA obtained by the method, a host cell comprising the RNA, and use of the method in the treatment of a disease.

Abstract: Disclosed are a LEAPER technique-based nucleic acid drug and a method for treating diseases such as Hurler syndrome by means of using the nucleic acid drug to target and edit RNA. The method comprises: performing an editing from adenosine base to hypoxanthine base on RNA by using the nucleic acid drug to precisely repair the pathogenic G>A mutation site of, for example, Hurler syndrome; thereby recovering the normal in vivo expression of a protein encoded by RNA, such as IDUA.

Abstract: The present application provides methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA. The present application further provides deaminase-recruiting RNAs used in the RNA editing methods and compositions comprising the same.

Abstract: In one embodiment, a system and method for partitioning a region for point cloud registration of LIDAR poses of an autonomous driving vehicle (ADV) using a regional iterative closest point (ICP) algorithm is disclosed. The method determines the frame pair size of one or more pairs of related LIDAR poses of a region of an HD map to be constructed. If the frame pair size is greater than a threshold, the region is further divided into multiple clusters. The method may perform the ICP algorithm for each cluster. Inside a cluster, the ICP algorithm focuses on a partial subset of the decision variables and assumes the rest of the decision variables are fixed. To construct the HD map, the method may determine if the results of the ICP algorithms from the clusters converge. If the solutions converge, a solution to the point cloud registration for the region is found.

Abstract: The present application discloses a high-precision mapping method and device, which relates to the field of autonomous driving. A specific implementation includes: acquiring global initial poses of multiple point clouds, where the point clouds are point clouds of a location for which a map is to be built and are collected by a lidar using a multi-circle collection mode; dividing the multiple point clouds into multiple spatial submap graphs according to a spatial distribution relationship of the multiple point clouds; optimizing, for each spatial submap graph, global initial poses of point clouds belonging to the spatial submap graph to acquire global poses of the point clouds in each spatial submap graph; and stitching the multiple spatial submap graphs together according to global poses of the point clouds in the multiple spatial submap graphs to acquire a base graph of the map to be built.

Abstract: A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.

Abstract: In one embodiment, a system is disclosed for registration of point clouds for autonomous driving vehicles (ADV). The system receives a number of point clouds and corresponding poses from ADVs equipped with LIDAR sensors capturing point clouds of a navigable area to be mapped, where the point clouds correspond to a first coordinate system. The system partitions the point clouds and the corresponding poses into one or more loop partitions based on navigable loop information captured by the point clouds. For each of the loop partitions, the system applies an optimization model to point clouds corresponding to the loop partition to register the point clouds. They system merges the one or more loop partitions together using a pose graph algorithm, where the merged partitions of point clouds are utilized to perceive a driving environment surrounding the ADV.

Abstract: Provided is a method for deaminating targeted cytosine in a target RNA of a cell. The method comprises: introducing, into the cell, a modified adenosine deaminase protein or a catalytic domain thereof, or a construct expressing the modified adenosine deaminase protein or the catalytic domain thereof, and a construct comprising an arRNA oligonucleotide that recruits the modified adenosine deaminase protein or the catalytic domain thereof to the target RNA or expressing arRNA. Further provided are an engineered composition or system for RNA editing, and a use of treating a disease by using the engineered composition or system to correct a T-C mutation.

Abstract: Provided is a method for targeted editing of target RNA containing a G to A mutation in a USH2A gene transcript based on LEAPER technology, comprising: introducing a construct of an adenosine deaminase recruiting RNA (arRNA) for editing the target RNA or a construct encoding said arRNA into a cell, wherein the arRNA comprises a complementary RNA sequence that hybridizes to the target RNA, and wherein the arRNA is capable of recruiting adenosine deaminase acting on RNA (ADAR), so that the target adenosine in the target RNA is deaminated, thereby performing in vivo editing of the base from A to I on RNA safely and effectively, repairing a pathogenic mutation site, and achieving the purpose of treating disease such as Usher syndrome.

Abstract: The present application relates to an LEAPER-based method for targeted editing of RNA, which comprises: safely and effectively performing adenosine-to-inosine RNA editing (A-to-I RNA editing) by LEAPER in vivo, thereby the pathogenic mutation sites can be accurately repaired, and all diseases caused by G>A mutations, such as MPS IH, can be treated.

Abstract: A method for purifying a universal human CAR-T cell, comprising: (i) destroying, by using gene editing technology, a TRAC gene region from position 23016448 to position 23016490 of chromosome 14 and a B2M gene region from position 45003745 to position 4500378 of chromosome 15 in the human CAR-T cell; (ii) then introducing a mRNA targeting TCR?/? into the gene-edited CAR-T cell; and (iii) in vitro culturing the CAR-T cell population after the described treatment.

Abstract: A novel preparation method for a universal CAR-T cell targeting a T-cell lymphoma cell, the universal CAR-T cell prepared by means of the method and a biological product comprising the universal CAR-T cell. The preparation method for the universal CAR-T cell targeting a T-cell lymphoma cell comprises: obtaining a T cell from a human donor having a healthy lymphatic system, and then disrupting a TRAC genome region and a B2M genome region in the T cell by means of a gene editing technology, so that CAR molecules targeting the T-cell lymphoma cell TCR?/? are stably expressed in the T cell. The universal CAR-T cell targeting a T-cell lymphoma cell prepared by means of the preparation method eliminates the natural TCR expression of a T cell, greatly reduces the graft-versus-host reaction, and meanwhile, also greatly reduces the immunogenicity thereof, and can continuously and efficiently kill the T-cell lymphoma cell.

Abstract: Provided are small molecule inhibitors for amplifying hematopoietic stem cells (HSCs) and a combination thereof. The small molecule inhibitors and the combination thereof can maintain the sternness of hematopoietic stem cells while promoting the in vitro amplification of hematopoietic stem cells (HSCs).

Abstract: In one embodiment, a system and method for point cloud registration of LIDAR poses of an autonomous driving vehicle (ADV) is disclosed. The method selects poses of the point clouds that possess higher confidence level during the data capture phase as fixed anchor poses. The fixed anchor points are used to estimate and optimize the poses of non-anchor poses during point cloud registration. The method may partition the points clouds into blocks to perform the ICP algorithm for each block in parallel by minimizing the cost function of the bundle adjustment equation updated with a regularity term. The regularity term may measure the difference between current estimates of the poses and previous or the initial estimates. The method may also minimize the bundle adjustment equation updated with a regularity term when solving the pose graph problem to merge the optimized poses from the blocks to make connections between the blocks.

Abstract: Disclosed is a method for determining the suitability of a cell therapy for an individual. By means of using a bioinformatic method and an experimental analysis method for an unbiased potential off-target site, a potential off-target site of a genetically modified cell from an individual is predicted before administering a cell therapy to an individual, and the potential off-target site is monitored after administering, to the individual, the genetically modified cell from the individual to determine whether gene editing occurs at the potential off-target site and to evaluate the suitability of the therapy on the basis of the occurrence of a gene editing event.

Abstract: A tunable mass damping device, including a connecting, a weighted assembly, and a top connecting assembly connecting an upper end of the connecting rod with a tower beam of a tower and including an anti-rotation mechanism. The anti-rotation mechanism includes: a laterally extending fixing plate fixedly connected with the tower beam through a longitudinally extending supporting member; a laterally extending first movable plate arranged under the fixing plate and fixedly connected with the upper end of the connecting rod; and two connecting shafts spaced apart from each other. Each of the connecting shafts has a lower end fixedly connected to the first movable plate, and an upper end inserted into the fixing plate and connected therewith through a connecting head. The connecting head includes an intermediate elastic layer arranged between inner and outer sleeves of the connecting head.

Abstract: In one embodiment, a system receives a stream of frames of point clouds from one or more LIDAR sensors of an ADV and corresponding poses in real-time. The system extracts segment information for each frame of the stream based on geometric or spatial attributes of points in the frame, where the segment information includes one or more segments of at least a first frame corresponding to a first pose. The system registers the stream of frames based on the segment information. The system generates a first point cloud map for the stream of frames based on the frame registration.