Abstract: A novel microfluidic sample holder has at least one sample receiving compartment for a sample fluid, at least one distributor channel which is linked with at least one sample receiving compartment, at least one distributor channel extending from every sample receiving compartment, at least one reaction chamber to which optionally one inlet channel branched off from the at least one distributor channel leads, and at least one vent opening for every reaction chamber. The sample holders are mainly used in microbiological diagnostics, immunology, PCR, clinical chemistry, microanalytics and/or the inspection of active substances. Methods for analyzing a sample substance using the sample holder and to kits including the sample holder as also included.