Abstract: In some embodiments there is provided a computer-implemented method for sorting cells. The method comprises receiving data comprising measurement datapoints for a plurality of cells, selecting a subset of the measurement datapoints using a region-of-interest, classifying the selected subset of measurement datapoints, and sorting the plurality of cells based on the classifying. In some embodiments, the method further comprises clustering previously received measurement datapoints and updating the region-of-interest based on the clustering by adjusting thresholds for the measurement datapoints.

Abstract: The present application discloses a plurality of embodiments and associated inventions, with respect to microfluidic systems for at least one of identifying, imaging, orientating, and sorting particles, in particular, biological cells, and more particularly, X and Y sperm cells. In some embodiments, a module system with functional connectors is provided, each module being connected by a connector that can provide additional functionality aside from enabling fluid flow between modules. The present disclosure also is directed to microfluidic systems which include particle delivery tubes configured to orient particles (e.g., X and Y sperm cells), as well as microfluidic systems for generating a static, spatial patterns within the microfluidic channel.