Patents by Inventor Peter Meinhold
Peter Meinhold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9145549Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: GrantFiled: June 2, 2014Date of Patent: September 29, 2015Assignee: The California Institute of TechnologyInventors: Frances H. Arnold, Matthew W. Peters, Peter Meinhold
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Publication number: 20150259710Abstract: The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize ?-ketoisovalerate as a substrate in various beneficial enzymatic conversions.Type: ApplicationFiled: July 30, 2012Publication date: September 17, 2015Applicant: Gevo, Inc.Inventors: Catherine Asleson Dundon, Kevin Roberg-Perez, Christopher Snow, Peter Meinhold
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Publication number: 20150218596Abstract: The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: September 10, 2014Publication date: August 6, 2015Inventors: Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano, Peter Meinhold, Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith
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Patent number: 9074178Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: GrantFiled: May 5, 2014Date of Patent: July 7, 2015Assignee: The California Institute of TechnologyInventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
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Patent number: 9012189Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: February 11, 2011Date of Patent: April 21, 2015Assignees: Gevo, Inc., The California Institute of TechnologyInventors: Sabine Bastian, Frances Arnold, Peter Meinhold
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Publication number: 20150010976Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: ApplicationFiled: June 2, 2014Publication date: January 8, 2015Inventors: Frances H. Arnold, Matthew W. Peters, Peter Meinhold
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Publication number: 20150004668Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: ApplicationFiled: May 5, 2014Publication date: January 1, 2015Applicant: The California Institute of TechnologyInventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M.Y. Chen
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Patent number: 8895272Abstract: Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.Type: GrantFiled: April 10, 2013Date of Patent: November 25, 2014Assignee: GEVO, Inc.Inventors: Andrew C. Hawkins, David A. Glassner, Thomas Buelter, James L. Wade, Peter Meinhold, Matthew W. Peters, Patrick R. Gruber, William A. Evanko, Aristos A. Aristidou, Marco Landwehr
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Publication number: 20140308721Abstract: The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: January 17, 2014Publication date: October 16, 2014Applicant: Gevo, Inc.Inventors: Jun Urano, Catherine Asleson Dundon, Peter Meinhold, Reid M. Renny Feldman, Aristos A. Aristidou, Andrew Hawkins, Thomas Buelter, Matthew Peters, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Ruth Berry, Ishmeet Kalra
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Publication number: 20140295512Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.Type: ApplicationFiled: June 15, 2012Publication date: October 2, 2014Applicants: CALIFORNIA INSTITUTE OF TECHNOLOGY, GEVO, INC.Inventors: Thomas Buelter, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Peter Meinhold, Ruth Berry, Christopher Snow, Sabine Bastian
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Publication number: 20140295513Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.Type: ApplicationFiled: July 11, 2012Publication date: October 2, 2014Applicants: CALIFORNIA INSTITUTE OF TECHNOLOGY, GEVO, INC.Inventors: Peter Meinhold, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Christopher Snow, Sabine Bastian, Sebastian Schoof
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Publication number: 20140212953Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: September 19, 2013Publication date: July 31, 2014Applicant: GEVO, Inc.Inventors: Thomas BUELTER, Andrew Hawkins, Stephanie Porter-Scheinman, Peter Meinhold, Catherine Asleson Dundon, Aristos Aristidou, Jun Urano, Doug Lies, Matthew Peters, Melissa Dey, Justas Jancauskas, Kent Evans, Julie Kelly, Ruth Berry
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Patent number: 8741616Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: GrantFiled: November 9, 2012Date of Patent: June 3, 2014Assignee: California Institute of TechnologyInventors: Frances H. Arnold, Matthew W. Peters, Peter Meinhold
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Patent number: 8715988Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: GrantFiled: April 6, 2007Date of Patent: May 6, 2014Assignee: California Institute of TechnologyInventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
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Publication number: 20140080188Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of an acetolactate-derived by-product such as 2,3-butanediol. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: March 9, 2012Publication date: March 20, 2014Applicant: GEVO, Inc.Inventors: Christopher Smith, Thomas Buelter, Peter Meinhold, Aristos Aristidou, Stephanie Porter-Scheinman
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Publication number: 20140017748Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: March 27, 2012Publication date: January 16, 2014Applicants: GEVO, INC., The California Institute of TechnologyInventors: Sabine BASTIAN, Frances Arnold, Peter Meinhold
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Patent number: 8614077Abstract: This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.Type: GrantFiled: November 22, 2011Date of Patent: December 24, 2013Assignee: Gevo, Inc.Inventors: William A. Evanko, Aharon M. Eyal, David A. Glassner, Fudu Miao, Aristos A. Aristidou, Kent Evans, Patrick R. Gruber, Andrew C. Hawkins, Peter Meinhold, Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano
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Publication number: 20130273622Abstract: Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.Type: ApplicationFiled: April 10, 2013Publication date: October 17, 2013Inventors: Andrew C. Hawkins, David A. Glassner, Thomas Buelter, James L. Wade, Peter Meinhold, Matthew W. Peters, Patrick R. Gruber, William A. Evanko, Aristos A. Aristidou, Marco Landwehr
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Publication number: 20130252298Abstract: The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: May 31, 2013Publication date: September 26, 2013Applicant: GEVO, INC.Inventors: Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano, Peter Meinhold, Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith
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Publication number: 20130183730Abstract: This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.Type: ApplicationFiled: November 22, 2011Publication date: July 18, 2013Applicant: GENO, INC.Inventors: William A. Evanko, Aharon M. Eyal, David A. Glassner, Fudu Miao, Aristos A. Aristidou, Kent Evans, Patrick R. Gruber, Andrew C. Hawkins, Peter Meinhold, Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano