Abstract: Multiple microbial host genetic systems with various molecular constructs enabling subcellular targeting, fusion protein generation and co-expression of ancillary enzymatic activities are used in the expression of potato proteinase inhibitor II. The expression levels achieved exceed previous reports by up to 10 to 1000 fold.

Abstract: The present invention provides polynucleotides and related polypeptides of a newly discovered serratia metalloprotease (SMP), as well as polynucleotides and related polypeptides of a newly discovered serratia metalloprotease inhibitor (SmaPI). The present invention further relates broadly to the use of the newly discovered SMP as a nematocidal agent, either as an administered agent, or as part of an integrated management system. The polynucleotides can be used to transform plants normally susceptible to free-living or burrowing plant parasitic nematodes, such as those of the genera Ascaris and Radopholus.

Abstract: A method of producing from sources of microorganisms enzymes having multiple improved characteristics. Soil samples are collected from a wide variety of sources that are diverse geographically and environmentally. The microorganisms are screened using microbiological screens to select microorganisms that produce enzymes that are active over a broad range of temperatures. Enzymes from selected microorganisms are screened for improved thermostability, extended active temperature range, extended active pH range, extended shelf-life, and improved protease resistance.