Abstract: A dental isolation tray has an outer side wall, an inner side wall, and a roof which joins the inner and outer side walls. An aspiration conduit having spaced apart aspiration ports is molded into the peripheral edges of the side walls and into the interface between the interior side wall and the roof. An aspiration coupling is provided for fluidly connecting the aspiration conduit with a suction tube. A portion of the roof and outer side wall is pre-cut to expose the occlusal and buccal aspects of preselected teeth. An adjustable bite block is mounted on or adjacent to the roof of the tray and extends substantially perpendicular to the roof of the tray. The full arch tray may have one or two quadrants cut-out and may be placed on either the upper or the lower arch to completely cover the arch and associated alveolar ridge. A single quadrant tray may be formed as either a left hand tray or a right hand tray and can also be placed over either upper or lower arches.