Patents by Inventor Phillip T. Olson
Phillip T. Olson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10415045Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: GrantFiled: September 9, 2015Date of Patent: September 17, 2019Assignee: DANISCO US INCInventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes
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Publication number: 20150376629Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: ApplicationFiled: September 9, 2015Publication date: December 31, 2015Inventors: Peter J. PUNT, Richard Paul BURLINGAME, Christine M. PYNNONEN, Phillip T. OLSON, Jan WERY, Johannes Heinrich VISSER, Mark A. EMALFARB, Jacob VISSER, Jan Cornelis VERDOES, Jacoba VERDOES
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Patent number: 9175296Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: GrantFiled: March 16, 2010Date of Patent: November 3, 2015Assignee: DYADIC NEDERLAND B.V.Inventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes, Jacoba Verdoes
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Publication number: 20120107856Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: ApplicationFiled: March 16, 2010Publication date: May 3, 2012Inventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cormelis Verdoes, Jacoba Verdoes
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Patent number: 5591438Abstract: A process for the recovery of high purity natamycin from fermentaion broth by extraction with methanol is disclosed. In one embodiment the process comprises the steps:(1) adding methanol to a natamycin feed stream comprising at least 2 g/L of solid suspended natamycin to form an extraction medium, and maintaining the extraction medium at a temperature of 0.degree.-25.degree. C., preferably not over 15.degree. C.;(2) adjusting the pH of the extraction medium to 1.0 to 4.5 while maintaining the temperature at 0.degree.-25.degree. C. for 0.5 to 30 hours, but preferably not over 0.5 hours when the temperature is 15.degree.-25.degree. C.;(3) removing solids from the extraction medium to form an extraction liquor;(4) raising the pH of the extraction liquor to 6.0-9.0; and(5) recovering precipitated natamycin.Type: GrantFiled: May 3, 1994Date of Patent: January 7, 1997Assignee: Bio-Technical Resources L.P.Inventors: Phillip T. Olson, James R. Millis, Michael H. Reimer
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Patent number: 5231014Abstract: A natamycin producing Streptomyces species ferments to produce natamycin. A spore suspension of the Streptomyces species is propagated in a predetermined medium to obtain a quantity of Streptomyces cells. The Streptomyces cells ferment in a predetermined production medium having a controlled pH to produce a recoverable amount of natamycin.Type: GrantFiled: August 5, 1991Date of Patent: July 27, 1993Assignee: Bio-Technical ResourcesInventors: Michael A. Eisenschink, Phillip T. Olson