Abstract: The invention relates to a device intended to break at least one closure element located inside a flexible tube, said closure element having a first part and a second part, said parts being separated by a weakening zone, it being possible to break said weakening zone in order to enable fluid to flow inside said flexible tube, said device comprising an assembly for holding said flexible tube, said assembly comprising a fixed element provided with a first housing intended to hold a first portion of said flexible tube, and a mobile element provided with a second housing intended to hold a second portion of said flexible tube, the two housings being aligned along a center line defining a neutral position of the mobile element, said device comprising a member for moving the mobile element to either side of its neutral position so as to be able to break the weakening zone of the closure element.

Abstract: The invention relates to a device intended to break at least one closure element located inside a flexible tube, said closure element having a first part and a second part, said parts being separated by a weakening zone, it being possible to break said weakening zone in order to enable fluid to flow inside said flexible tube, said device comprising an assembly for holding said flexible tube, said assembly comprising a fixed element provided with a first housing intended to hold a first portion of said flexible tube, and a mobile element provided with a second housing intended to hold a second portion of said flexible tube, the two housings being aligned along a center line defining a neutral position of the mobile element, said device comprising a member for moving the mobile element to either side of its neutral position so as to be able to break the weakening zone of the closure element.

Abstract: Methods for modifying the physical, chemical and/or biological properties of a fluid by irradiation are provided. In one embodiment, a method comprising supplying a flow of a fluid to an irradiation chamber; focusing the flow of the fluid so as to create at least one fluid layer; and applying radiation to the fluid layer in a defined portion of the irradiation chamber thereby modifying at least one physical, chemical or biological property of the fluid layer is provided. Systems for the implementation of such methods are also provided.

Abstract: Methods for modifying the physical, chemical and/or biological properties of a fluid by irradiation are provided. In one embodiment, a method comprising supplying a flow of a fluid to an irradiation chamber; focusing the flow of the fluid so as to create at least one fluid layer; and applying radiation to the fluid layer in a defined portion of the irradiation chamber thereby modifying at least one physical, chemical or biological property of the fluid layer is provided. Systems for the implementation of such methods are also provided.

Abstract: A method for expanding and/or preserving cells inside a culture vessel containing a culture medium provides for the renewal of the culture medium by a culture medium stream, at least a portion of which comes from a culture medium volume contained in a gas-enrichment vessel of the culture medium, the enrichment-vessel further containing a gas volume which is separated from the culture medium volume by a free interface, the gas volume being renewed by a gas stream that is introduced into the enrichment vessel directly into the gas volume, the flow of the stream being arranged so as to enable a gas exchange between the culture medium volume and the gas volume at the interface thereof.

Abstract: Methods for modifying the physical, chemical and/or biological properties of a fluid by irradiation are provided. In one embodiment, a method comprising supplying a flow of a fluid to an irradiation chamber; focusing the flow of the fluid so as to create at least one fluid layer; and applying radiation to the fluid layer in a defined portion of the irradiation chamber thereby modifying at least one physical, chemical or biological property of the fluid layer is provided. Systems for the implementation of such methods are also provided.