Abstract: Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.

Abstract: The disclosure provides a freezing device for freezing a liquid mixture and a method using the same. The freezing device includes a container and a tray removably disposed in the container. The tray has a tray plate disposed above a container bottom wall and has a plurality of spaced-apart receptacles for receiving the liquid mixture. When liquid nitrogen is introduced into a region between the tray plate and a container bottom wall, the liquid mixture received in the receptacles is solidified by the liquid nitrogen and is formed into shaped solid coolant pieces.

Abstract: Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.

Abstract: Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.

Abstract: Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.

Abstract: A laminated sheet includes a polymer layer selected from the group consisting of polyalkylene phthalate, polyalkylene isophthalate, polyalkylene terephthalate, and combinations thereof, and two poly(?,?,??,??-tetrafluoro-para-xylylene) layers respectively connected to two opposite surfaces of the polymer layer.