Patents by Inventor Ponnampalam Gopalakrishnakone
Ponnampalam Gopalakrishnakone has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7579433Abstract: The present invention provides a new 18-residue homodimerized peptide, designated PIP [59-67] dimer (SEQ ID NO: 1), which is a mutant of the optimized anti-inflammatory peptide P-NT.II, the patent for which has recently been filed [1]. P-NT.II has the potential to modulate both the inflammatory and bone damaging components of rheumatoid arthritis, and was originally designed on the basis of the primary structure of the anti-inflammatory protein termed ‘Phospholipase Inhibitor from Python (PIP)’ [2]. Using solid phase chemistry, variants of P-NT.II were designed and examined for inhibitory activity against secretory phospholipase A2 (sPLA2), a key enzyme involved in the inflammatory pathway, and matrix metalloproteinases (MMPs) that are involved in the remodeling and degradation of the extracellular matrix in rheumatoid arthritis (RA) and cancer. Among the family of mutants tested, the dimerized peptide was found to be the most potent inhibitor against sPLA2 as well as the human recombinant MMP-1.Type: GrantFiled: April 28, 2006Date of Patent: August 25, 2009Assignee: National University of SingaporeInventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kazuki Sato
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Patent number: 7422890Abstract: A phospholipase A2 inhibitor protein designated “Phospholipase Inhibitor from Python” (PIP)—formerly designated “Python Antitoxic Factor” (PAF)—is given by SEQ ID NO:2. The partial amino acid sequence for PIP was initially determined from the native protein purified from the blood serum of a non-venomous snake, Python reticulatus. The complete PIP polynucleotide sequence was obtained from a cDNA clone encoding PIP, given by SEQ ID NO:1, along with the full amino acid sequence deduced from it. Also disclosed is a recombinant protein PIP, which shows strong lethal toxin neutralizing activity similar to the native PIP, and has potent anti-inflammatory activity. Both the native and the functionally equivalent recombinant PIP are useful for the prevention or treatment of conditions such as snakebites, insect stings, and inflammatory diseases.Type: GrantFiled: November 30, 2005Date of Patent: September 9, 2008Assignee: National University of SingaporeInventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kandiah Jeyaseelan, Arunmozhiarasi Armugam
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Publication number: 20070128179Abstract: The present invention provides a method for the treatment and/or prevention of a bacterial related condition comprising administering to a subject a therapeutically effective amount of at least one phospholipase, isoform, derivative, mutant and/or fragment thereof. The phospholipase, isoform, derivative, mutant and/or fragment thereof, may be obtained from at least one venom selected from the group consisting of: Daboia russelli russelli, Daboia russelli siamensis, Daboia russelli pulchella, Crotalus adamanteus, Crotalus durissus terrificus, Pseudechis australis, Agkistrodon halys, Pseudechis guttata, Bitis arietans, Bitis gabonica rhinoceros, Echis carinatus, Acanthopis antarticus, Bungarus candidus, Bothrops asper, Bothrops jararacussu and/or Apis mellifera. The present invention also provides isolated peptides comprising at least one amino acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, an isoform, derivative, mutant and/or fragment thereof.Type: ApplicationFiled: November 8, 2006Publication date: June 7, 2007Inventors: Ponnampalam Gopalakrishnakone, Ramar Samy
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Publication number: 20070037253Abstract: The present invention provides a new 18-residue homodimerized peptide, designated PIP [59-67] dimer (SEQ ID NO: 1), which is a mutant of the optimized anti-inflammatory peptide P-NT.II, the patent for which has recently been filed [1]. P-NT.II has the potential to modulate both the inflammatory and bone damaging components of rheumatoid arthritis, and was originally designed on the basis of the primary structure of the anti-inflammatory protein termed ‘Phospholipase Inhibitor from Python (PIP)’ [2]. Using solid phase chemistry, variants of P-NT.II were designed and examined for inhibitory activity against secretory phospholipase A2 (sPLA2), a key enzyme involved in the inflammatory pathway, and matrix metalloproteinases (MMPS) that are involved in the remodeling and degradation of the extracellular matrix in rheumatoid arthritis (RA) and cancer. Among the family of mutants tested, the dimerized peptide was found to be the most potent inhibitor against sPLA2 as well as the human recombinant MMP-1.Type: ApplicationFiled: April 28, 2006Publication date: February 15, 2007Inventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kazuki Sato
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Patent number: 7176281Abstract: Peptides that have potent PLA2-inhibitory activity are disclosed. Homology searches of known PLA inhibitory molecules were used to identify and subsequently design potent peptide molecules that can induce neuroprotective as well as anti-inflammatory effect. These peptides were shown to protect against degeneration of joints in transgenic mouse model prone to arthritis. Protection from kainate-induced excitotoxic neuronal injury was also observed using these peptides. These peptides, their analogs and derivatives have potential as neuroprotective and/or anti-inflammatory agents in a clinical setting.Type: GrantFiled: April 30, 2004Date of Patent: February 13, 2007Assignee: National University of SingaporeInventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Wei-Yi Ong, Kazuki Sato
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Publication number: 20060222638Abstract: An isolated peptide comprising the amino acid sequence of SEQ ID NO: 2, or a variant, derivative and/or fragment thereof having the function of HMGCoA reductase inhibitor, phosphomevalonate inhibitor, reducing the accumulation of cholesterol in the cholesterol biosynthesis pathway and/or reducing the level of serum cholesterol. Also disclosed is a pharmaceutical composition comprising the peptide having sequence SEQ ID NO:2 or a variant, derivative and/or fragment thereof. Also disclosed is a method for treatment or prophylaxis of disorders characterised by the accumulation of cholesterol, its by-products and/or related lipid derived products, comprising administering to a subject in need at least one peptide comprising the amino acid sequence of SEQ ID NO: 2, or a variant, derivative and/or fragment thereof.Type: ApplicationFiled: June 4, 2004Publication date: October 5, 2006Applicant: National University of SingaporeInventors: Kandiah Jeyaseelan, Arunmozhiarasi Armugam, Siaw Chai, Prabhakaran Ramkishen, Ponnampalam Gopalakrishnakone, Kwon Huat Tan
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Patent number: 7094575Abstract: A phospholipase A2 inhibitor protein designated “Phospholipase Inhibitor from Python” (PIP)—formerly designated “Python Antitoxic Factor” (PAF)—is given by SEQ ID NO:2. The partial amino acid sequence for PIP was initially determined from the native protein purified from the blood serum of a non-venomous snake, Python reticulatus. The complete PIP polynucleotide sequence was obtained from a cDNA clone encoding PIP, given by SEQ ID NO:1, along with the full amino acid sequence deduced from it. Also disclosed is a recombinant protein PIP, which shows strong lethal toxin neutralizing activity similar to the native PIP, and has potent anti-inflammatory activity. Both the native and the functionally equivalent recombinant PIP are useful for the prevention or treatment of conditions such as snakebites, insect stings, and inflammatory diseases.Type: GrantFiled: June 7, 2002Date of Patent: August 22, 2006Assignee: National University of SingaporeInventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kandiah Jeyaseelan, Arunmozhiarasi Armugam
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Publication number: 20060160100Abstract: There is provided an electrochemical assay method for detecting a target molecule, for example a protein, in a sample, which involves the use of a protective monolayer and a redox polymer to form a bilayer immobilized on an electrode. The monolayer protects the electrode from non-specific adherence of reagents, particular proteins, to the electrode while simultaneously providing a surface that can be functionalized to immobilize a capture molecule and that can interact with the redox polymer.Type: ApplicationFiled: January 19, 2005Publication date: July 20, 2006Inventors: Zhiqiang Gao, Ponnampalam Gopalakrishnakone, Van Le, Fang Xie
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Publication number: 20060078551Abstract: A phospholipase A2 inhibitor protein designated “Phospholipase Inhibitor from Python” (PIP)—formerly designated “Python Antitoxic Factor” (PAF)—is given by SEQ ID NO:2. The partial amino acid sequence for PIP was initially determined from the native protein purified from the blood serum of a non-venomous snake, Python reticulatus. The complete PIP polynucleotide sequence was obtained from a cDNA clone encoding PIP, given by SEQ ID NO:1, along with the full amino acid sequence deduced from it. Also disclosed is a recombinant protein PIP, which shows strong lethal toxin neutralizing activity similar to the native PIP, and has potent anti-inflammatory activity. Both the native and the functionally equivalent recombinant PIP are useful for the prevention or treatment of conditions such as snakebites, insect stings, and inflammatory diseases.Type: ApplicationFiled: November 30, 2005Publication date: April 13, 2006Inventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kandiah Jey Aseelan, Arunmozhiarasi Armugam
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Publication number: 20050069530Abstract: Peptides that have potent PLA2-inhibitory activity are disclosed. Homology searches of known PLA inhibitory molecules were used to identify and subsequently design potent peptide molecules that can induce neuroprotective as well as anti-inflammatory effect. These peptides were shown to protect against degeneration of joints in transgenic mouse model prone to arthritis. Protection from kainate-induced excitotoxic neuronal injury was also observed using these peptides. These peptides, their analogs and derivatives have potential as neuroprotective and/or anti-inflammatory agents in a clinical setting.Type: ApplicationFiled: April 30, 2004Publication date: March 31, 2005Inventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Wei-Yi Ong, Kazuki Sato
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Patent number: 6613745Abstract: The present invention relates generally to peptide molecules and to derivatives, homologues, analogues and mimetics thereof capable of inducing or facilitating analgesia or partial analgesia alone or in combination with other analgesic molecules. The present invention also contemplates a method of inducing or facilitating analgesia or partial analgesia by the administration of a peptide or a derivative, homologue, analogue or mimetic thereof. The amino acid sequence of the peptide molecules of the present invention are derived from or based on amino acid sequences of snake venom toxins, and, in particular, &agr;-neurotoxins.Type: GrantFiled: July 26, 2000Date of Patent: September 2, 2003Assignee: National University of SingaporeInventors: Ponnampalam Gopalakrishnakone, Xiao Chun Pu, Peter Tsun-Hon Wong, Mathew Choon Eng Gwee, R. Manjunatha Kini
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Publication number: 20030027764Abstract: A phospholipase A2 inhibitor protein designated “Phospholipase Inhibitor from Python” (PIP)—formerly designated “Python Antitoxic Factor” (PAF)—is given by SEQ ID NO:2. The partial amino acid sequence for PIP was initially determined from the native protein purified from the blood serum of a non-venomous snake, Python reticulatus. The complete PIP polynucleotide sequence was obtained from a cDNA clone encoding PIP, given by SEQ ID NO:1, along with the full amino acid sequence deduced from it. Also disclosed is a recombinant protein PIP, which shows strong lethal toxin neutralizing activity similar to the native PIP, and has potent anti-inflammatory activity. Both the native and the functionally equivalent recombinant PIP are useful for the prevention or treatment of conditions such as snakebites, insect stings, and inflammatory diseases.Type: ApplicationFiled: June 7, 2002Publication date: February 6, 2003Inventors: Ponnampalam Gopalakrishnakone, Maung-Maung Thwin, Kandiah Jeyaseelan, Arunmozhiarasi Armugam