Abstract: A method for optogenetics experiments, based on wavefront shaping and including: calculating the transmission matrix between an input end and an output end of the multimode fiber under a fixed shape; implanting the output end into an intracranial space of an experimental subject; and performing wavefront compensation to a light to be input into the input end, according to the spatial position of the optical stimulation and the transmission matrix of the multimode fiber, to form a compensated expanded light, and inputting the compensated expanded light from the input end into the multimode fiber, such that the compensated expanded light, after being transmitted by the multimode fiber to the output end and output from the output end, is capable of focusing at the spatial position of the optical stimulation.

Abstract: Systems and methods for focusing a light pulse within a focus area using nonlinear photoacoustic wavefront shaping (PAWS) are disclosed herein. The method includes modulating a spatial phase pattern of a light pulse's waveform based on a Grueneisen-relaxation photoacoustic (GR-PA) feedback signal. In addition, systems and methods for performing Grueneisen-relaxation photoacoustic microscopy (GR-PAM) are disclosed herein that include analyzing photoacoustic signals resulting from illumination of a focus region by two closely spaced light pulses. A method of obtaining an absorption coefficient of a sample using Grueneisen-relaxation photoacoustic microscopy (GR-PAM) is also disclosed.

Abstract: A method for optogenetics experiments, based on wavefront shaping and including: calculating the transmission matrix between an input end and an output end of the multimode fiber under a fixed shape; implanting the output end into an intracranial space of an experimental subject; and performing wavefront compensation to a light to be input into the input end, according to the spatial position of the optical stimulation and the transmission matrix of the multimode fiber, to form a compensated expanded light, and inputting the compensated expanded light from the input end into the multimode fiber, such that the compensated expanded light, after being transmitted by the multimode fiber to the output end and output from the output end, is capable of focusing at the spatial position of the optical stimulation.

Abstract: Systems and methods for focusing a light pulse within a focus area using nonlinear photoacoustic wavefront shaping (PAWS) are disclosed herein. The method includes modulating a spatial phase pattern of a light pulse's waveform based on a Grueneisen-relaxation photoacoustic (GR-PA) feedback signal. In addition, systems and methods for performing Grueneisen-relaxation photoacoustic microscopy (GR-PAM) are disclosed herein that include analyzing photoacoustic signals resulting from illumination of a focus region by two closely spaced light pulses. A method of obtaining an absorption coefficient of a sample using Grueneisen-relaxation photoacoustic microscopy (GR-PAM) is also disclosed.