Abstract: Provided is a multi-functional integrated molecular test cartridge and application thereof. The multi-functional integrated molecular test cartridge includes a deformable assembly, a blocking assembly and a reaction assembly, where the blocking assembly is placed between the deformable assembly and the reaction assembly, and a channel in communication with the reaction assembly and the deformable assembly is formed on the blocking assembly. The multi-functional integrated molecular test cartridge can be used for isothermal amplification reactions such as cross priming amplification (CPA), recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA), can achieve integrated pre-loading of all reagents, a whole-process integrated test from a sample to a result, and flexible sample processing modes, and has high biological safety and a low risk of product and sample leakage.

Abstract: An nucleic acid integrated detection reagent tube is provided, it includes a main tube and one or more branch tubes, wherein a lysing zone, a cleaning zone and a plurality of separation plugs comprising at least a least a separation plug and a second separation plug which are sequentially disposed, the first separation plug is used for separating the lysing zone and the cleaning zone, a reaction zone is provided in the branch tubes and the second separation plug is positioned at a connection between the branch tubes and the main tube or inside the branch tubes; hydrophobic layers in a liquid or a solid phase disposed at each of the plurality of separation plugs; and a magnetic bead channel for magnetically carrying the nucleic acid to sequentially pass through each hydrophobic layer penetrating to the branch tubes defined in an inner wall of the detection reagent tube.

Abstract: An nucleic acid integrated detection method is provided, the method includes separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A biological sample processing device includes a containing bottle. A receiving opening is provided and a liquid outlet is provided. The device further includes an upper cover assembly and a lower cover. The upper cover assembly is connected to the upper end of the containing bottle. The lower cover is connected to the lower end of the containing bottle. The upper cover assembly includes an upper cover, a lower seal, a piston and a puncturing element. The upper cover is provided with a through hole, and the lower seal is disposed at a lower end of the through hole. The piston is closely matched with an inner wall of the through hole and can slide along the inner wall to drive the puncturing element to pierce the lower seal. The disclosure provides advantages that the biological sample can be processed by the provided device without opening the upper cover.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: The present invention relates to methods of amplification of nucleic acid sequences; more particularly, it relates to methods of amplifying target sequences by utilizing cross priming isothermal amplification. The present invention relates to methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence. The present invention also relates to reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases.

Abstract: The present invention relates to methods of amplification of nucleic acid sequences; more particularly, it relates to methods of amplifying target sequences by utilizing cross priming isothermal amplification. The present invention relates to methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence. The present invention also relates to reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases.

Abstract: The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection.

Abstract: The present invention relates to methods of amplification of nucleic acid sequences; more particularly, it relates to methods of amplifying target sequences by utilizing cross priming isothermal amplification. The present invention relates to methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence. The present invention also relates to reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases.

Abstract: The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection.

Abstract: The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection.

Abstract: Methods of amplifying target sequences by utilizing cross priming isothermal amplification are disclosed Methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence are also disclosed Reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases are also disclosed.

Abstract: The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection.

Abstract: The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection.

Abstract: The present disclosure provides efficient and reproducible methods for individually synthesizing oligomers in a parallel manner (e.g., oligonucleotides) on a solid support to produce pools of oligomers. Pools of oligonucleotides can be used for a variety of genomic and proteomic applications, including synthesis of genes or long DNA of any arbitrary sequence, PCR template amplification, and to generate primers for multiplexing PCR or transcription. Rapid availability of these oligonucleotide products will greatly accelerate the processes of de novo protein design, vaccine development, production of short RNA fragments, such as siRNA, oligonucleotide-based drug screening, and SNP sample preparation.

Abstract: Primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). Disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of HIV nucleic acids.

Abstract: Primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). Disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of HIV nucleic acids.

Abstract: Disclosed herein is a newly-identified DNA sequence from Mycobacterium kansasii designated KATS2. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the detection of M. kansasii nucleic acids. M. kansasii-specific methods, probes, amplification primers, and kits are preferred.

Abstract: Amplification primers and methods for specific amplification and detection of a Yersinia enterocolitica target are disclosed. The primer-target binding sequences are useful for amplification and detection of Y. enterocolitica target in a variety of amplification and detection reactions.