Abstract: The present invention relates to a method for detecting nucleic acids by fluorescent PCR. The method combines primer-activated polymerization reaction and specific fluorescence-labeled probe, which detects the target nucleic acid with high selectivity and high specificity.

Abstract: The present invention relates to a method for detecting nucleic acids by fluorescent PCR. The method combines primer-activated polymerization reaction and specific fluorescence-labeled probe, which detects the target nucleic acid with high selectivity and high specificity.

Abstract: A method and apparatus for sign-bill return based on a blockchain, a device and a readable storage medium, through acquiring (S101) a sign-bill return request sent by a shipment terminal; acquiring (S102) pre-stored target sign-bill information according to the sign-bill return request; acquiring (S103) a target hash value corresponding to the target sign-bill information from the blockchain; and sending (S104) the target sign-bill information to the shipment terminal, when verifying that the target sign-bill information is correct by the target hash value, the tamper-proof blockchain storage of the target sign-bill information is achieved, which not only improves efficiency of the sign-bill return, but also improves reliability of the sign-bill return.

Abstract: A switch driving circuit for driving a full-controlled power switch is disclosed, including a pulse-width modulation unit, a pulse transformer, an anti-interference module, a pulse-width demodulation unit and a driving-power amplifier. The pulse-width modulation unit converts a driving input signal into a positive-negative narrow pulse-width signal. The anti-interference module is coupled to the secondary side and the positive-negative narrow pulse-width signal includes multiple positive pulses and negative pulses. The anti-interference module filters out the ones from the positive pulses and negative pulses of which the pulse amplitude does not reach an effective threshold, and meanwhile the anti-interference module suppress a common-mode noise caused by a high voltage transient variation at a moment when the full-controlled power switch is turned on or off. The pulse-width demodulation unit converts the filtered positive-negative narrow pulse-width signal into a driving output signal.

Abstract: A switch driving circuit for driving a full-controlled power switch is disclosed, including a pulse-width modulation unit, a pulse transformer, an anti-interference module, a pulse-width demodulation unit and a driving-power amplifier. The pulse-width modulation unit converts a driving input signal into a positive-negative narrow pulse-width signal. The anti-interference module is coupled to the secondary side and the positive-negative narrow pulse-width signal includes multiple positive pulses and negative pulses. The anti-interference module filters out the ones from the positive pulses and negative pulses of which the pulse amplitude does not reach an effective threshold, and meanwhile the anti-interference module suppress a common-mode noise caused by a high voltage transient variation at a moment when the full-controlled power switch is turned on or off. The pulse-width demodulation unit converts the filtered positive-negative narrow pulse-width signal into a driving output signal.