Abstract: A high-throughput optical sectioning three-dimensional imaging system which includes: a light beam modulation module configured to modulate a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens; an imaging module configured to employ a camera to image, in different rows of pixels, a sample under illumination of the modulated light beam; a cutting module configured to cut off an imaged surface layer of the sample; a demodulation module configured to demodulate a sample image of one sample strip of one surface layer into an optical sectioning image, and reconstruct the optical sectioning image of each sample strip of each surface layer into a three-dimensional image. The present disclosure achieves imaging of a whole sample by dividing the sample into at least one surface layer, dividing the at least one surface layer into at least one sample strip, and imaging each sample strip.

Abstract: A high-throughput optical sectioning imaging method and imaging system. The method includes: modulating a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens, the modulated light beam having incompletely identical modulated intensities on the focal plane of the objective lens; imaging, in different rows of pixels, a sample under illumination of the modulated light beam to obtain sample images in the different rows of pixels; obtaining focal plane images of sample images in the different rows of pixels by demodulation of the sample images according to a demodulation algorithm. The system includes a light beam modulation module, an imaging module and a demodulation module.

Abstract: A tomographic imaging method which includes the steps of activating fluorescence in a surface layer of a protein-marked or fluorescent dye-marked biological tissue sample not emitting fluorescence or only emitting specific fluorescence to acquire an activated surface of biological tissue sample; performing fluorescence excitation on the acquired surface biological tissue sample, and imaging the fluorescence to acquire a fluorescence image of the surface layer; cutting off the surface layer; exposing an inactivated new surface layer after cutting the surface layer; repeatedly performing activating, imaging and cutting off steps for the new surface layer to repeat tomographic imaging in such a manner till acquiring a two-dimensional image of each layer of the biological tissue sample; and overlapping the two-dimensional images to acquire a complete three-dimensional image of the biological tissue sample, thus acquiring three-dimensional structure information of the entire sample.

Abstract: A high-throughput optical sectioning three-dimensional imaging system which includes: a light beam modulation module configured to modulate a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens; an imaging module configured to employ a camera to image, in different rows of pixels, a sample under illumination of the modulated light beam; a cutting module configured to cut off an imaged surface layer of the sample; a demodulation module configured to demodulate a sample image of one sample strip of one surface layer into an optical sectioning image, and reconstruct the optical sectioning image of each sample strip of each surface layer into a three-dimensional image. The present disclosure achieves imaging of a whole sample by dividing the sample into at least one surface layer, dividing the at least one surface layer into at least one sample strip, and imaging each sample strip.

Abstract: A high-throughput optical sectioning imaging method and imaging system. The method includes: modulating a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens, the modulated light beam having incompletely identical modulated intensities on the focal plane of the objective lens; imaging, in different rows of pixels, a sample under illumination of the modulated light beam to obtain sample images in the different rows of pixels; obtaining focal plane images of sample images in the different rows of pixels by demodulation of the sample images according to a demodulation algorithm. The system includes a light beam modulation module, an imaging module and a demodulation module.

Abstract: The light control device includes a light modulation module and a dispersion compensation module. The light modulation module is used for modulating an incident light field to obtain a target diffraction light field. The dispersion compensation module is used for performing dispersion compensation on the target diffraction light field, so that light fields having different wavelength in the target diffraction light field have the same spatial location distribution, or the light fields having different wavelength in the target diffraction light field have the same spatial angle distribution.

Abstract: A tomographic imaging method which includes the steps of activating fluorescence in a surface layer of a protein-marked or fluorescent dye-marked biological tissue sample not emitting fluorescence or only emitting specific fluorescence to acquire an activated surface of biological tissue sample; performing fluorescence excitation on the acquired surface biological tissue sample, and imaging the fluorescence to acquire a fluorescence image of the surface layer; cutting off the surface layer; exposing an inactivated new surface layer after cutting the surface layer; repeatedly performing activating, imaging and cutting off steps for the new surface layer to repeat tomographic imaging in such a manner till acquiring a two-dimensional image of each layer of the biological tissue sample; and overlapping the two-dimensional images to acquire a complete three-dimensional image of the biological tissue sample, thus acquiring three-dimensional structure information of the entire sample.

Abstract: The light control device includes a light modulation module and a dispersion compensation module. The light modulation module is used for modulating an incident light field to obtain a target diffraction light field. The dispersion compensation module is used for performing dispersion compensation on the target diffraction light field, so that light fields having different wavelength in the target diffraction light field have the same spatial location distribution, or the light fields having different wavelength in the target diffraction light field have the same spatial angle distribution.

Abstract: An automatic slicing and collecting device, the device including: a first tank, a vibrating microtome, a first pipeline, and a second pipeline. The first tank is filled with a buffer solution. The vibrating microtome is disposed in the first tank. One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump. The pump includes a reversible motor. The first pipeline is provided with a first valve, and a filter is disposed between the pump and the first pipeline. One end of the second pipeline is disposed between the first valve of the first pipeline and the pump, and the second pipeline is provided with a second valve.

Abstract: An automatic slicing and collecting device, the device including: a first tank, a vibrating microtome, a first pipeline, and a second pipeline. The first tank is filled with a buffer solution. The vibrating microtome is disposed in the first tank. One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump. The pump includes a reversible motor. The first pipeline is provided with a first valve, and a filter is disposed between the pump and the first pipeline. One end of the second pipeline is disposed between the first valve of the first pipeline and the pump, and the second pipeline is provided with a second valve.

Abstract: Disclosed is a dual-targeted therapeutic peptide for nasopharyngeal carcinoma formed by covalently linking a targeted therapeutic peptide for nasopharyngeal carcinoma, a peptide linker and a targeted therapeutic peptide with an ?-helical structure for nasopharyngeal carcinoma. Also disclosed is a nanoparticle containing the peptide. The peptide and the nanoparticle can be used to treat nasopharyngeal carcinoma.

Abstract: Disclosed is a dual-targeted therapeutic peptide for nasopharyngeal carcinoma formed by covalently linking a targeted therapeutic peptide for nasopharyngeal carcinoma, a peptide linker and a targeted therapeutic peptide with an ?-helical structure for nasopharyngeal carcinoma. Also disclosed is a nanoparticle containing the peptide. The peptide and the nanoparticle can be used to treat nasopharyngeal carcinoma.

Abstract: A light pulse positioning apparatus with dispersion compensation includes an acousto-optical device and a dispersive element optically coupled thereto. The dispersive element is placed and oriented in relation to the acousto-optical device to spatially and temporally disperse the light pulse and thus compensate, respectively, a spatial dispersion and a temporal dispersion caused by the acousto-optical device. The acousto-optical device can include one or more acousto-optical deflectors for one-dimensional or two-dimensional laser pulse positioning. The dispersive element can be a prism placed in front of the acousto-optical device. In a two-dimensional configuration, a single prism, if properly oriented, is sufficient for dispersion compensation of both acousto-optical deflectors.

Abstract: A light pulse positioning apparatus with dispersion compensation includes an acousto-optical device and a dispersive element optically coupled thereto. The dispersive element is placed and oriented in relation to the acousto-optical device to spatially and temporally disperse the light pulse and thus compensate, respectively, a spatial dispersion and a temporal dispersion caused by the acousto-optical device. The acousto-optical device can include one or more acousto-optical deflectors for one-dimensional or two-dimensional laser pulse positioning. The dispersive element can be a prism placed in front of the acousto-optical device. In a two-dimensional configuration, a single prism, if properly oriented, is sufficient for dispersion compensation of both acousto-optical deflectors.

Abstract: An optical method and system for in vivo, non-invasive imaging of biological tissue includes a stimulator and a spectrophotometer. The stimulator is constructed and arranged to stimulate cognition in a subject. The spectrophotometer is co-operatively arranged with the stimulator. The spectrophotometer is coupled to an optical module constructed to provide a multiplicity of arrayed source-detector pairs constructed for direct engagement with the subject. The system includes a light source constructed to introduce electromagnetic radiation of a visible or infra-red wavelength into biological tissue and a light detector constructed to detect optical radiation that has migrated in the tissue.

Abstract: An optical system for in vivo, non-invasive imaging of tissue change including an optical module, a spectrophotometer, and a processor. The optical module includes an array of input ports and detection ports located in a selected geometrical pattern to provide a multiplicity of arrayed source-detector pairs engaged directly with the subject. The spectrophotometer includes at least one light source constructed to introduce electromagnetic radiation of visible or infra-red wavelength into the examined tissue successively at the input ports, wherein the wavelength is sensitive to a constituent of the imaged tissue, and at least one detector constructed to detect, at the detection ports, radiation of the selected wavelength that has migrated in the tissue from respective input ports.