Abstract: Provided are a humanized antibody specifically binding to EpCAM, and a chimeric antigen receptor, a nucleic acid, a vector, a cell, and the use associated with the humanized antibody. The humanized antibody comprises a heavy chain variable region and a light chain variable region. The sequence of the heavy chain variable region comprises a sequence shown in SEQ ID.1, a sequence shown in SEQ ID.2 and a sequence shown in SEQ ID.3. The sequence of the light chain variable region comprises a sequence shown in SEQ ID.4, a sequence shown in SEQ ID.5, and a sequence shown in SEQ ID.6. After the humanized antibody is constructed as a chimeric antigen receptor-T Cell, EpCAM-positive tumor cells can be specifically identified and killed, and tumors can be cleared in an animal body.

Abstract: The use of an immune killer cell against circulating tumor cells in solid tumor treatment, which comprises transfusing the immune killer cell into peripheral blood of a subject to act on the circulating tumor cells in peripheral blood. In the use of a solid tumor treatment, the immune killer cell can act on the circulating tumor cells by means of transfusing an effective dose of the immune killer cell into the peripheral blood of the subject, which is beneficial for blocking solid tumor metastasis by means of inhibiting the colonization of circulating tumor cells in tissue and improving the survival period of the subject.

Abstract: The present invention discloses a preparation method of instant bone milk, including: washing edible animal bones and then crushing, adding a certain amount composite effect enhancer, performing two high pressure hydrolysis-instantaneous pressure relief in high pressure hydrolysis chamber, collecting liquid-phase materials, performing centrifugation for degreasing, wet sieving, homogenizing, spray drying to obtain powdered instant bone milk. A device for preparation method of instant bone milk comprises a high-pressure hydrolysis tank, a grading conveying pipe and a gas-liquid separator. The instant bone milk prepared by the present invention has stable chemical structure, uniform particle size distribution and regular normal distribution, high protein solubility and good solution stability. And the preparation method has simple process, low cost equipment and good versatility.

Abstract: The present invention discloses a preparation method of instant bone milk, including: washing edible animal bones and then crushing, adding a certain amount composite effect enhancer, performing two high pressure hydrolysis-instantaneous pressure relief in high pressure hydrolysis chamber, collecting liquid-phase materials, performing centrifugation for degreasing, wet sieving, homogenizing, spray drying to obtain powdered instant bone milk. A device for preparation method of instant bone milk comprises a high-pressure hydrolysis tank, a grading conveying pipe and a gas-liquid separator. The instant bone milk prepared by the present invention has stable chemical structure, uniform particle size distribution and regular normal distribution, high protein solubility and good solution stability. And the preparation method has simple process, low cost equipment and good versatility.