Abstract: A method of creating a mathematical model which is employed in the determination of at least one work location in a multi-layered laminated circuit panel. The mathematical model for modifying drill data takes into consideration translational and rotational compensations caused by the encountered stretching or shrinking of the multi-layered panel subsequent to pressing or due to thermally processing, whereby the mathematical model may be utilized to modify drill data in order to accurately predict hole or via locations.

Abstract: A novel rolling cone rock bit includes a plurality of PDC or other cutters mounted to the leg of the drill bit and positioned to cut the troublesome corner of the bottomhole. The plurality of cutters may be the primary cutting component at gage diameter, or may be redundant to gage teeth on a rolling cutter that cut to gage diameter. Consequently, the occurrence of undergage drilling from the wear and failure of the gage row on a rolling cutter is lessened. Another inventive feature is the inclusion of a mud ramp that creates a large junk slot from the borehole bottom up the drill bit. The resulting pumping action of the drill bit ramp speeds up the removal of chips or drilling cuttings from the bottom of the borehole, reduces the level of hydrostatic pressure at the bottom of the borehole and minimizes the wearing effect of cone inserts regrinding damaging drill cuttings.

Abstract: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample.

Abstract: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample.

Abstract: A voltage reference circuit receives an input voltage through a first port and a time varying input signal through a second port. The voltage reference circuit includes a switching circuit that is responsive to the first and the second ports and that generates an AC signal from the input voltage. The voltage reference circuit further includes a voltage multiplier circuit, coupled to switching circuit that receives the AC signal and creates a DC signal with a selected voltage level. The voltage reference circuit further includes a voltage regulator, coupled to the voltage multiplier circuit that regulates the DC signal from the voltage multiplier circuit. An output voltage is provided through an output port.

Abstract: Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Caged RNAs capable of repressing translation of a target mRNA or silencing transcription of a target gene are also provided, along with related methods, systems, and kits. Methods and compositions for introducing RNAs into cells, using RNAs covalently associated with protein transduction domains and/or lipids, are provided. Also provided are methods and compositions for selectively attenuating expression of a target mRNA by controlling expression of an interfering RNA, an RNA capable of initiating translation repression, or an RNA capable of initiating transcriptional silencing.

Abstract: A drill bit configures hydraulic streams from the bit nozzles for the particular cutting structure to be cleaned based on the cutting structure location and geometry. The projected impingement location for each hydraulic stream projected fluid path is customized for a corresponding roller cone, with each nozzle or nozzle receptacle being individually positioned according to improve cone cleaning. At least two nozzles or nozzle receptacles should differ on the drill bit.

Abstract: A structure and method to establish an electrical connection between a tester and an electrical component. A flexible dielectric layer has a first side and a second side. A through via extends through the first side and the second side of the dielectric layer. A blind via is placed in a position that is offset from the through via and extends laterally in a first direction from a section of the first through via to a section of the flexible dielectric layer. The blind via extends in a second direction from the first side of the flexible dielectric layer to a section of the flexible dielectric layer that is between the first side and the second side of the dielectric layer. An electrically conductive member extends through the through via and extends into the blind via, thereby filling the through via and the blind via. The electrically conductive member has a first surface and a second surface.

Abstract: Disclosed are a variety of arcuate-shaped inserts for drill bits, and in particular, for placement in rolling cone cutters of drill bits. The arcuate inserts include 360° or ring-shaped inserts, as well as inserts of smaller arcuate length. The arcuate inserts are suitable for use in all surfaces of the rolling cone cutter, and in other locations in drill bits, and may have specialized cutting surfaces and material enhancements to enhance their cutting duty performance. Certain arcuate inserts may include stress relieving discontinuities such that, upon assembly into the cone or during drilling, the arcuate inserts may fragment in a controlled and predicted manner into shorter arcuate lengths.

Abstract: Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Methods and compositions for introducing interfering RNAs into cells, using RNAs covalently associated with protein transduction domains and/or lipids, are provided. Also provided are methods and compositions for selectively attenuating expression of a target mRNA by controlling expression of an interfering RNA.

Abstract: Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

Abstract: A method for producing small pitch z-axis electrical interconnections in layers of dielectric materials which are applied to printed wiring boards and diverse electronic packages. A method for parallel fabrication of intermediate structures which are subsequently jointed to form a final structure. In addition there is provided a z-interconnected electrical structure, employing dielectric materials such as resin coated copper, employable in the manufacture of diverse type of electronic packages, including printed wiring boards (PWBs), substrates, multi-chip modules and the like.

Abstract: A number of caged (e.g., photoactivatable) compositions are provided, for example, caged enzyme sensors, caged binding sensors, caged nucleic acid probes, caged interfering RNAs, caged antisense nucleic acids, caged ribozymes, caged biomolecular analogs, caged transcription factors, caged antibodies, caged molecular decoys, and caged aptamers. Compositions comprising a plurality of caged components are also provided, as are labeled and optionally caged modulators. Also provided are kits for making the caged compositions and methods for making and for using the caged compositions. Related systems and devices are also provided (e.g., an uncaging device that can trigger photoactivatable assays and optionally detect signals from the assays).

Abstract: Systems and methods of biochemical analysis are provided. A method of performing biochemical assays includes loading a first plurality of reservoirs on a first liquid carrier with a first plurality of compounds, coupling a second carrier with the first carrier, the second carrier having a second plurality of reservoirs configured to couple with the first plurality of reservoirs and containing a second plurality of compounds, transferring at least a portion of the first plurality of compounds to the second plurality of reservoirs, and separating at least one component from the second plurality of reservoirs. A system for biochemical analysis includes a first carrier and a second carrier. The first carrier includes a first substrate and a plurality of reservoirs in the substrate for retaining a first plurality of compounds.

Abstract: Various functions for maintenance, calibration and validation of an automated instrument that is capable of performing multiple simultaneous assays using fluorescence detection and microscopic beads as a solid phase are provided by a single block that is inserted into the instrument in place of a sample plate. The block contains several different sets of wells, all appropriately labeled, for the retention of various liquids and bead suspensions, including different sets for the different functions.

Abstract: Fusion proteins in which glutathione S-transferase (GST) is a fusion partner are used as an immobilized binding member in screening procedures or other multi-analyte test procedures based on protein interaction. In such procedures, a particular protein is selected from a group of candidate proteins on the basis of the binding affinity of that protein for a target protein, with either the candidate proteins or the target protein being a fusion partner with GST and the GST portion of the fusion partner having been immobilized on glutathione-coated particles by the binding of GST to glutathione. The particles themselves are classifiable by different values of a differentiation parameter that permits them to be distinguished by flow cytometry, and the procedure is conducted in a manner that associates the individual candidate proteins with individual classes of the particles.

Abstract: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample.

Abstract: Fusion proteins in which glutathione S-transferase (GST) is a fusion partner are used as an immobilized binding member in screening procedures or other multi-analyte test procedures based on protein interaction. In such procedures, a particular protein is selected from a group of candidate proteins on the basis of the binding affinity of that protein for a target protein, with either the candidate proteins or the target protein being a fusion partner with GST and the GST portion of the fusion partner having been immobilized on glutathione-coated particles by the binding of GST to glutathione. The particles themselves are classifiable by different values of a differentiation parameter that permits them to be distinguished by flow cytometry, and the procedure is conducted in a manner that associates the individual candidate proteins with individual classes of the particles.

Abstract: The present invention provides standard diluents for use in multiplex assays comprising a biological fluid that normally includes two or more different target analytes but that is substantially free of the two or more different target analytes, as well as kits and methods relating to the standard diluent. The standard diluents of the invention provide an accurate and reliable means to calibrate the amount of multiple target analytes in a single assay.

Abstract: Fusion proteins in which glutathione S-transferase (GST) is a fusion partner are used as an immobilized binding member in screening procedures or other multi-analyte test procedures based on protein interaction. In such procedures, a particular protein is selected from a group of candidate proteins on the basis of the binding affinity of that protein for a target protein, with either the candidate proteins or the target protein being a fusion partner with GST and the GST portion of the fusion partner having been immobilized on glutathione-coated particles by the binding of GST to glutathione. The particles themselves are classifiable by different values of a differentiation parameter that permits them to be distinguished by flow cytometry, and the procedure is conducted in a manner that associates the individual candidate proteins with individual classes of the particles.