Abstract: The invention relates to an optical arrangement and a method for correcting centration errors and/or angle errors in a beam path. The beam path here comprises an optical compensated system in which at least two optical elements are present and aligned relative to one another such that imaging aberrations of the optical elements are compensated. According to the invention, a correction unit is arranged in an infinity space of the beam path and between the at least two optical elements, wherein the correction unit changes the propagation direction of radiation propagating along the beam path and the correction unit either has a reflective surface or is embodied to be transmissive for the radiation. The correction unit is movable such that the angle of a change in the propagation direction can be set.

Abstract: An adapter is useful with a sample holder of a microscope. The adapter has at least one support region for reproducibly orienting the adapter in a mounted state in a sample holder support. The adapter also has a first coupling point for detachable connection to a coupling structure of a sample holder on one side, and a second coupling point for detachable connection to a coupling structure of a sample manipulator on a side of the adapter opposite to the first coupling point. The adapter is useful with the sample holder, a sample chamber, a microscope and a method for arranging a sample in a detection beam path of a microscope.

Abstract: A computer-implemented method for determining an appropriate control strategy for a mobile agent for an environment with one or more dynamic objects. The method includes: providing a number of different scenarios wherein to each of the scenarios a number of dynamic objects is associated, wherein for each of the scenarios, each of the dynamic objects is associated with a start, a goal and a behavior specification; providing a number of control strategy candidates for the mobile agent; benchmarking each of the control strategy candidates in any of the scenarios; selecting the control strategy for the mobile agent depending on the result of the benchmarking of the control strategy candidates.

Abstract: An adapter is useful with a sample holder of a microscope. The adapter has at least one support region for reproducibly orienting the adapter in a mounted state in a sample holder support. The adapter also has a first coupling point for detachable connection to a coupling structure of a sample holder on one side, and a second coupling point for detachable connection to a coupling structure of a sample manipulator on a side of the adapter opposite to the first coupling point. The adapter is useful with the sample holder, a sample chamber, a microscope and a method for arranging a sample in a detection beam path of a microscope.

Abstract: The invention relates to an optical arrangement and a method for correcting centration errors and/or angle errors in a beam path. The beam path here comprises an optical compensated system in which at least two optical elements are present and aligned relative to one another such that imaging aberrations of the optical elements are compensated. According to the invention, a correction unit is arranged in an infinity space of the beam path and between the at least two optical elements, wherein the correction unit changes the propagation direction of radiation propagating along the beam path and the correction unit either has a reflective surface or is embodied to be transmissive for the radiation. The correction unit is movable such that the angle of a change in the propagation direction can be set.

Abstract: Laser Scanning Microscope with a non-descanned detection and/or observation beam path, wherein a beam splitter is provided for the separation of the illumination and the detection beam paths. In the direction of the detection, at least one optical arrangement is provided for the regular transmission of the detected light, whereby between the beam splitter and the optical system, an additional optical arrangement is provided for reducing the diameter of the bundle of the beam to be imaged.

Abstract: An arrangement in the illumination beam path of a laser scanning microscope, comprising a mirror which can be introduced into the beam path for coupling in at least one additional wavelength, and a method for the operation of a laser scanning microscope with a mirror which can be swiveled in rapidly for coupling at least one additional wavelength into the illumination beam path, wherein the swiveling process is synchronized with the illumination control by a control unit in such a way that at least one additional wavelength is coupled into the illumination beam path when the mirror is swiveled out.

Abstract: Laser scanning microscope with at least four separate input-coupling locations for wavelength ranges in the UV region, in the visible region and in the IR region, and advantageously with at least two individually displaceable collimators for achieving a maximum flexibility in the selection of dyes and evaluating methods, preferably for displaying cellular calcium and the associated receptors in living tissue over long periods of time (time lapse) with infrared lasers and UV lasers, wherein fluorescence detection and uncaging are applied, for releasing drugs (uncaging) and IR illumination with transmission detection, for the realization of applications with fluorescing proteins of the (G)FP family, including photoactivation of GFP and CFP/YFP-FRET, for the use of CY 5.5 dyes in the dark-red region (laser 675 nm) for tumor research.

Abstract: An arrangement for direct input coupling of a laser, preferably of a short-pulse laser, for nonlinear sample excitation which is located outside of the microscope housing, into the beam path of a laser scanning microscope (LSM), comprising a housing in which is integrated an adjusting laser that can be coupled into the microscope beam path by beam splitters, wherein the housing is advantageously integrated so as to be insertable in the scan module of the LSMA, and a beam splitter for the direct coupling laser has high transmission in the direction of the objective and, further, partial transmission for back-reflections of the adjusting laser coming from the specimen for adjusting the overlapping of the adjusting laser and direct coupling laser.

Abstract: An arrangement in the illumination beam path of a laser scanning microscope, comprising a mirror which can be introduced into the beam path for coupling in at least one additional wavelength, and a method for the operation of a laser scanning microscope with a mirror which can be swiveled in rapidly for coupling at least one additional wavelength into the illumination beam path, wherein the swiveling process is synchronized with the illumination control by a control unit in such a way that at least one additional wavelength is coupled into the illumination beam path when the mirror is swiveled out.

Abstract: A method and arrangement are disclosed for increasing the depth contrast in microscope imaging. The method and implementation described can be designated as structured illumination for generating quasi-confocal optical sections. In implementing the method, a grating structure located in the field diaphragm plane of a microscope, the object plane and the TV intermediate image plane of a microscope are arranged confocally. The term “confocally” refers to the fact that the grating, object and the intermediate image plane are positioned on optically conjugate planes. By this arrangement, the grating structure is projected in the object plane of the microscope and the object which is structured in this way is imaged in the TV intermediate image plane of the microscope by the optical system following it. Optical sections are generated by calculating the modulation depth of the structured object.

Abstract: Near-field optical microscope with a probe tip which is arranged on one side of a light-transmitting specimen and moved in a scanning manner. The probe tip serves as a point light source. Optics for collecting light transmitted through the specimen and transmitting it to a detection unit or for collecting illumination light are provided on the other side of the specimen. The movement of the probe tip is adapted to on the detection side, or the probe serves to detect specimen light. A detection unit is arranged following the probe in the direction of illumination, and a scanning illumination adapted to the probe movement is carried out on the other side of the specimen.

Abstract: The invention relates to a method for increasing the depth of contrast during microscopic imaging. The method and implementation described can be designated as structured illumination, which creates quasi-confocal optical sections. During the implementation of the method, a grid structure, located in the illumination field stop plane of a microscope, the object plane and the TV intermediate-image plane of a microscope are arranged “confocally”. This assembly enables the grid structure to be projected into the object plane of the microscope and the object, thus structured, to be reproduced on the TV intermediate-image plane using the following optical system. Optical sections are created by the calculation of the modulation depth of the structured object. The object is reproduced perpendicular to the direction of observation on several focus planes, to achieve a 3-dimensional registration of said object and is detected using an array-detector (e.g. CCD camera).

Abstract: An illumination device for a light microscope comprises a scanning microscope probe which is integrated in the center thereof. A scanning probe microscope comprising such illumination device is also disclosed.

Abstract: An illumination device for a light microscope comprises a scanning microscope probe which is integrated in the center thereof. A scanning probe microscope comprising such illumination device is also disclosed.

Abstract: The invention relates to the combined use of ketoprofen and specific inorganic basic substances with an improved quality of action.