Abstract: Provided herein are IL-2 muteins, IL-2 mutein Fc-fusion molecules, anti-IL-2 antibodies, and complexes comprising an anti IL-2 antibody bound to an IL-2 cytokine that preferentially expand and activate T regulatory cells and are amenable to large scale production. Also provided herein are variant human IgG1 Fc molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at N297. Also provided herein are linker peptides that are glycosylated when expressed in mammalian cells. Also provided herein are methods of making and using the compositions of the present invention.

Abstract: Described herein are compositions and methods related to antigen binding proteins that bind to human ST2, including antibodies. In particular embodiments, the disclosure provides fully human anti-ST2 antibodies and deriviatives and variants thereof. Further provided are nucleic acids encoding such antibodies and antibody fragments, variants, and derivatives. Also, provided are methods of making and using such antibodies including methods of treating and preventing autoimmune and inflammatory disorders.

Abstract: A method is disclosed for segmentation of three dimensional image data sets, to obtain digital models of objects identifiable in the image data set. The image data set may be obtained from any convenient source, including medical imaging modalities, geological imaging, industrial imaging, and the like. A graph cuts method is applied to the image data set, and a level set method is then applied to the data using the output from the graph cuts method. The graph cuts process comprises determining location information for the digital data on a 3D graph, and cutting the 3D graph to determine approximate membership information for the object. The boundaries of the object is then refined using the level set method. Finally, a representation of the object volumes can be derived from an output of the level set method. Such representation may be used to generate rapid prototyped physical models of the objects.