Patents by Inventor Ravi Kapur
Ravi Kapur has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240141424Abstract: The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.Type: ApplicationFiled: May 1, 2023Publication date: May 2, 2024Inventors: Roland Stoughton, Ravi Kapur, Barb Ariel Cohen, Mehmet Toner
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Publication number: 20240132969Abstract: This disclosure relates to new assay methods for analysis of circulating tumor cells (CTCs) in blood samples for detection, e.g., early detection, and/or monitoring of disease, e.g., cancer. The methods provide ultra-high sensitivity and specificity, and include the use of microfluidic isolation of CTCs and digital detection of RNA derived from the CTCs.Type: ApplicationFiled: December 26, 2023Publication date: April 25, 2024Inventors: Daniel A. Haber, Ravi Kapur, Mehmet Toner, Shyamala Maheswaran, Xin Hong, David Tomoaki Miyamoto, Tanya Todorova, Sarah Javaid
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Patent number: 11944971Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.Type: GrantFiled: April 30, 2021Date of Patent: April 2, 2024Assignee: The General Hospital CorporationInventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
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Patent number: 11944972Abstract: A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.Type: GrantFiled: June 1, 2021Date of Patent: April 2, 2024Assignee: The General Hospital CorporationInventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
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Patent number: 11898209Abstract: This disclosure relates to new assay methods for analysis of circulating tumor cells (CTCs) in blood samples for detection, e.g., early detection, and/or monitoring of disease, e.g., cancer. The methods provide ultra-high sensitivity and specificity, and include the use of microfluidic isolation of CTCs and digital detection of RNA derived from the CTCs.Type: GrantFiled: October 8, 2020Date of Patent: February 13, 2024Assignee: The General Hospital CorporationInventors: Daniel A. Haber, Ravi Kapur, Mehmet Toner, Shyamala Maheswaran, Xin Hong, David Tomoaki Miyamoto, Tanya Todorova, Sarah Javaid
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Patent number: 11781187Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.Type: GrantFiled: November 16, 2018Date of Patent: October 10, 2023Assignees: The General Hospital Corporation, GPB Scientific, LLC, Verinata Health, Inc.Inventors: Daniel Shoemaker, Mehmet Toner, Ravi Kapur, Roland B. Stoughton, Ronald W. Davis
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Patent number: 11725180Abstract: Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.Type: GrantFiled: October 15, 2021Date of Patent: August 15, 2023Assignee: The General Hospital CorporationInventors: Philipp S. Spuhler, Kyle C. Smith, Fabio Fachin, Thomas Alan Barber, Ravi Kapur, Mehmet Toner, Vincent Pai, Nezihi Murat Karabacak
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Patent number: 11674176Abstract: The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.Type: GrantFiled: June 30, 2020Date of Patent: June 13, 2023Assignees: Verinata Health, Inc, The General Hospital Corporation, GPB Scientific, LLCInventors: Roland Stoughton, Ravi Kapur, Barb Ariel Cohen, Mehmet Toner
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Publication number: 20230168160Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.Type: ApplicationFiled: June 27, 2022Publication date: June 1, 2023Inventors: Roland Stoughton, Ravi Kapur, Barb Ariel Cohen, Daniel Shoemaker, Ronald W. Davis, Mehmet Toner
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Publication number: 20230021752Abstract: The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g. fetal cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject, e.g. a fetus by analyzing a cellular sample from the subject.Type: ApplicationFiled: February 14, 2022Publication date: January 26, 2023Inventors: Ravi Kapur, Mehmet Toner, Zihua Wang, Martin Fuchs
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Patent number: 11446664Abstract: Extracting and concentrating particles from a first fluid sample includes: providing the first fluid sample to a fluid exchange module of a microfluidic device, providing a second fluid sample to the fluid exchange module, in which the first fluid sample and the second fluid sample are provided under conditions such that particle-free portions of the first fluid sample are shifted, and an inertial lift force causes the particles in the first fluid sample to cross streamlines and transfer into the second fluid sample; passing the second fluid sample containing the transferred particles to a particle concentration module under conditions such that particle-free portions of the second fluid sample are shifted, and such that the particles within the second fluid sample are focused to a streamline within the particle concentration module.Type: GrantFiled: January 6, 2020Date of Patent: September 20, 2022Assignee: The General Hospital CorporationInventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
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Patent number: 11378498Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.Type: GrantFiled: March 16, 2020Date of Patent: July 5, 2022Assignees: Verinata Health, Inc., The General Hospital Corporation, GPR Scientific, LLCInventors: Roland Stoughton, Ravi Kapur, Barb Ariel Cohen, Daniel Shoemaker, Ronald W. Davis, Mehmet Toner
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Publication number: 20220106553Abstract: Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.Type: ApplicationFiled: October 15, 2021Publication date: April 7, 2022Inventors: Philipp S. Spuhler, Kyle C. Smith, Fabio Fachin, Thomas Alan Barber, Ravi Kapur, Mehmet Toner, Vincent Pai, Nezihi Murat Karabacak
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Publication number: 20220074932Abstract: Methods of using particle size amplification to facilitate size-based particle separation and concentration. At least one of the methods includes introducing a plurality of binding moieties into a fluid sample; allowing at least one of the binding moieties to bind two or more biological particles to form a particle cluster, in which the particle cluster comprises a first type of biological particle bound to a second different type of biological particle; and flowing the fluid sample comprising the particle cluster into a particle sorting region of a microfluidic device.Type: ApplicationFiled: December 26, 2019Publication date: March 10, 2022Inventors: Jon F. Edd, Kaja Kaastrup, Ravi Kapur, Mehmet Toner
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Patent number: 11261492Abstract: The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g. fetal cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject, e.g. a fetus by analyzing a cellular sample from the subject.Type: GrantFiled: October 7, 2019Date of Patent: March 1, 2022Assignees: The General Hospital Corporation, Verinata Health, Inc., GPB Scientific, LLCInventors: Ravi Kapur, Mehmet Toner, Zihua Wang, Martin Fuchs
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Publication number: 20210370298Abstract: Methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood) include introducing a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).Type: ApplicationFiled: June 11, 2021Publication date: December 2, 2021Inventors: Mehmet Toner, George Truskey, Ravi Kapur
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Patent number: 11155779Abstract: Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.Type: GrantFiled: January 24, 2019Date of Patent: October 26, 2021Assignee: The General Hospital CorporationInventors: Philipp S. Spuhler, Kyle C. Smith, Fabio Fachin, Thomas Alan Barber, Ravi Kapur, Mehmet Toner, Vincent Pai, Nezihi Murat Karabacak
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Publication number: 20210283610Abstract: A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.Type: ApplicationFiled: June 1, 2021Publication date: September 16, 2021Inventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
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Publication number: 20210252514Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.Type: ApplicationFiled: April 30, 2021Publication date: August 19, 2021Inventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
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Patent number: 11052392Abstract: Methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood) include introducing a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).Type: GrantFiled: September 21, 2018Date of Patent: July 6, 2021Assignee: The General Hospital CorporationInventors: Mehmet Toner, George Truskey, Ravi Kapur