Abstract: A method of and apparatus for dispensing rubber gloves in an aseptic condition for insertion of the hands of a user. The gloves to be dispensed are contained within a chamber in a suitable housing provided with hand holes for a user. The gloves are attached to a continuous web of material provided with an aperture at each glove attachment for insertion of a hand of the user. Each glove is covered by a packaging film that is lightly perforated at each glove. The gloves are fed intermittently into registry with the hand holes in the housing. The user activates a vacuum pressure which is in fluid communication with the hand holes causing the gloves to expand and rupture through the film of packaging material. The user inserts his hands, deactivates the vacuum pressure, thrusts his hands further into the housing to detach a temporary retention of the gloves and removes his hands from the housing with the gloves aseptically applied.