Patents by Inventor Renata Rimseliéné
Renata Rimseliéné has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11560553Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: April 8, 2020Date of Patent: January 24, 2023Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Publication number: 20200325460Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: ApplicationFiled: April 8, 2020Publication date: October 15, 2020Inventors: Remigijus SKIRGAILA, Renata RIMSELIENE, Rasa SUKACKAITE, Martyna SIMUTYTE, Skaiste VALINSKYTE, Karolis MATJOSAITIS, Mindaugas UKANIS
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Patent number: 10626383Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: January 13, 2017Date of Patent: April 21, 2020Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Patent number: 10273530Abstract: This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.Type: GrantFiled: January 13, 2017Date of Patent: April 30, 2019Assignee: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Donata Ulcinaite, Birute Gagiliene, Rasa Sukackaite, Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Dovile Dekaminaviciute
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Publication number: 20170204384Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: ApplicationFiled: January 13, 2017Publication date: July 20, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Publication number: 20170204454Abstract: This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.Type: ApplicationFiled: January 13, 2017Publication date: July 20, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Donata Ulcinaite, Birute Gagiliene, Rasa Sukackaite, Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Dovile Dekaminaviciute
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Patent number: 9650675Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recognition sequence 5?-TCGA-3? are also disclosed.Type: GrantFiled: December 17, 2015Date of Patent: May 16, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Remigijus Skirgalia, Arvydas Lubys
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Publication number: 20170081646Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: ApplicationFiled: September 29, 2016Publication date: March 23, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubeleviciute, Renata Rimseliene, Sigitas Burinskas
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Publication number: 20160194706Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recognition sequence 5?-TCGA-3? are also disclosed.Type: ApplicationFiled: December 17, 2015Publication date: July 7, 2016Inventors: Renata Rimseliene, Remigijus Skirgalia, Arvydas Lubys
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Publication number: 20140011196Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recongition sequence 5?-TCGA-3? are also disclosed.Type: ApplicationFiled: July 1, 2013Publication date: January 9, 2014Inventors: Renata Rimseliene, Remigijus Skirgaila, Arvydas Lubys
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Publication number: 20130034879Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: ApplicationFiled: August 2, 2012Publication date: February 7, 2013Applicant: Fermentas UABInventors: Remigijus Skirgaila, Agne Tubeleviciute, Renata Rimseliéné, Sigitas Burinskas
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Patent number: 6893854Abstract: A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises: (a) mutagenising a polynucleotide encoding a restriction endonuclease with specificity for a recognition sequence so as to produce one or more mutated polynucleotides; and (b) isolating therefrom a polynucleotide encoding a mutated restriction endonuclease with specificity for an altered recognition sequence by selecting a polynucleotide which expresses a restriction endonuclease with methylase specificity for the altered recognition sequence.Type: GrantFiled: July 18, 2001Date of Patent: May 17, 2005Assignee: Fermentas UABInventors: Arvydas Janulaitis, Renata Rimseliene, Arvydas Lubys
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Publication number: 20030040614Abstract: A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises:Type: ApplicationFiled: July 18, 2001Publication date: February 27, 2003Applicant: Fermentas ABInventors: Arvydas Janulaitis, Renata Rimseliene, Arvydas Lubys