Patents by Inventor Renuka de Silva
Renuka de Silva has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220390459Abstract: Described herein are 1,2-dioxetanes that are useful as chemiluminescent probes, diagnostic agents, and imaging agents. Also described herein are compositions containing such compounds and methods of using the same.Type: ApplicationFiled: October 28, 2020Publication date: December 8, 2022Inventors: Khaledur Rashid, Guoping Wang, Barry A. Schoenfelner, Wenhua Xie, Renuka de Silva
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Patent number: 10703971Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.Type: GrantFiled: June 30, 2017Date of Patent: July 7, 2020Assignee: Beckman Coulter, Inc.Inventors: Renuka De Silva, Robert A. Eickholt, Jaya S. Koti, Mark D. Sandison, David L. Schumm, Wenhua Xie
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Publication number: 20190161675Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.Type: ApplicationFiled: June 30, 2017Publication date: May 30, 2019Applicant: Beckman Coulter, Inc.Inventors: Renuka DE SILVA, Robert A. EICKHOLT, Jaya S. KOTI, Mark D. SANDISON, David L. SCHUMM, Wenhua XIE
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Patent number: 10036708Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.Type: GrantFiled: April 2, 2015Date of Patent: July 31, 2018Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
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Publication number: 20150212005Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.Type: ApplicationFiled: April 2, 2015Publication date: July 30, 2015Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
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Patent number: 9029092Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.Type: GrantFiled: February 26, 2010Date of Patent: May 12, 2015Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
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Patent number: 8772501Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: GrantFiled: January 24, 2013Date of Patent: July 8, 2014Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder Gupta, Lakshmi S. Kaanumalle
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Patent number: 8389298Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.Type: GrantFiled: March 19, 2010Date of Patent: March 5, 2013Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Wenhua Xie
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Patent number: 8367842Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: GrantFiled: July 15, 2010Date of Patent: February 5, 2013Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder K. Gupta, Lakshmi S. Kaanumalle
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Patent number: 8106203Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.Type: GrantFiled: June 2, 2009Date of Patent: January 31, 2012Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
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Publication number: 20100273189Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample, the assay method comprising forming a reaction mixture in an aqueous solution, by adding a chemiluminescent-labeled immobilized specific binding member, an activator-labeled specific binding member, a selective signal inhibiting agent, and a sample, wherein the chemiluminescent-labeled immobilized specific binding member and activator-labeled specific binding member bind to analyte present in the sample to form a binding complex, and adding to the reaction mixture a trigger solution to release a detectable chemiluminescent signal correlated to the amount of the analyte-bound binding complex present in the reaction mixture.Type: ApplicationFiled: February 26, 2010Publication date: October 28, 2010Applicant: BECKMAN COULTER, INC.Inventors: Hashem AKHAVAN-TAFTI, Renuka DE SILVA, Terri MCLERNON, James MENDOZA, Michael SALVATI, Nir SHAPIR, Wenhua XIE
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Publication number: 20100267071Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.Type: ApplicationFiled: February 26, 2010Publication date: October 21, 2010Applicant: BECKMAN COULTER, INC.Inventors: Hashem AKHAVAN-TAFTI, Dean G. BINGER, Ying Chen, Renuka DE SILVA, Terri MCLERNON, James MENDOZA, Bruce H. ODEGAARD, Michael SALVATI, Nir SHAPIR, Wenhua XIE
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Publication number: 20100190271Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.Type: ApplicationFiled: March 19, 2010Publication date: July 29, 2010Applicant: Lumigen, Inc.Inventors: Hashem AKHAVAN-TAFTI, Renuka de Silva, Wenhua Xie
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Publication number: 20100173429Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.Type: ApplicationFiled: March 19, 2010Publication date: July 8, 2010Applicant: Lumigen, Inc.Inventors: Hashem AKHAVAN-TAFTI, Renuka De SILVA, Wenhua XIE
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Patent number: 7682839Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.Type: GrantFiled: March 16, 2007Date of Patent: March 23, 2010Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
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Publication number: 20090246819Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.Type: ApplicationFiled: June 2, 2009Publication date: October 1, 2009Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua XIE
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Patent number: 7560556Abstract: Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.Type: GrantFiled: March 4, 2005Date of Patent: July 14, 2009Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
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Publication number: 20070190526Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.Type: ApplicationFiled: February 15, 2007Publication date: August 16, 2007Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Robert A. Eickholt, Michael E. Mazelis, Wenhuas Xie, Richard S. Handley, Monica A. Bray, Michelle L. Mastronardi, Elizabeth A. O'Conner, Sarada Siripurapu
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Publication number: 20070172878Abstract: Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.Type: ApplicationFiled: March 16, 2007Publication date: July 26, 2007Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Wenhua Xie
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Patent number: RE49466Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.Type: GrantFiled: July 31, 2020Date of Patent: March 21, 2023Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka de Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie