Patents by Inventor Rhett Martineau
Rhett Martineau has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11345954Abstract: The invention provides methods for detecting specific nucleic acids using a loop-mediated isothermal amplification (LAMP) reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage multiple-threshold array (MTA). During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection.Type: GrantFiled: August 7, 2017Date of Patent: May 31, 2022Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Deirdre Meldrum
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Patent number: 11332782Abstract: Methods for detecting single nucleotide polymorphisms in nucleotide sequences using LAMP reactions are provided herein. Generally, two sets of LAMP primers, a wild-type primer that matches expected DNA sequences and an SNP primer that matches the expected SNP DNA are provided. One method includes providing the wild-type primer and the SNP primer in separate wells of a multi-well microfluidic array device, adding the sample nucleotide sequence into the wells seeded with the primers, and initiating LAMP reactions within the wells. The method includes observing the reaction differential between the primers and determining the status of the DNA with regard to that particular SNP. A second method includes providing the primers with tags in a mixture, adding the sample nucleotide sequence to the mixture, and initiating LAMP reactions.Type: GrantFiled: August 7, 2017Date of Patent: May 17, 2022Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Rhett Martineau, Deirdre Meldrum
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Publication number: 20200385794Abstract: The invention provides methods for detecting specific nucleic acids using a LAMP reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage MTA. During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection. Methods of the invention are especially useful in circumstances where there is lower or intermittent power supply.Type: ApplicationFiled: August 7, 2017Publication date: December 10, 2020Applicant: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Deirdre Meldrum
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Publication number: 20200063197Abstract: The present disclosure relates to real-time quantification using loop-mediated isothermal amplification, in particular real-time colorimetric reverse transcription quantitative loop-mediated isothermal amplification (RT-qLAMP). In some embodiments, RT-qLAMP is used to diagnose the presence of and also quantitate the amount of Zika virus in a sample.Type: ApplicationFiled: May 15, 2018Publication date: February 27, 2020Inventors: Deirdre Meldrum, Shufang Ci, Shih-Hui (Joseph) Chao, Rhett Martineau, Weimin Gao
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Patent number: 10471426Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: GrantFiled: July 13, 2018Date of Patent: November 12, 2019Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Publication number: 20190177784Abstract: Methods for detecting single nucleotide polymorphisms in nucleotide sequences using LAMP reactions are provided herein. Generally, two sets of LAMP primers, a wild-type primer that matches expected DNA sequences and an SNP primer that matches the expected SNP DNA are provided. One method includes providing the wild-type primer and the SNP primer in separate wells of a multi-well microfluidic array device, adding the sample nucleotide sequence into the wells seeded with the primers, and initiating LAMP reactions within the wells. The method includes observing the reaction differential between the primers and determining the status of the DNA with regard to that particular SNP. A second method includes providing the primers with tags in a mixture, adding the sample nucleotide sequence to the mixture, and initiating LAMP reactions.Type: ApplicationFiled: August 7, 2017Publication date: June 13, 2019Applicant: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Deirdre Meldrum
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Patent number: 10278617Abstract: A method is provided for sensing ammonia in a breath sample. The method comprises conditioning the breath sample so that the conditioned breath sample has a relative humidity greater than a relative humidity corresponding to a first inflection point of a downwardly concave lowest ammonia iso-concentration curve. It also comprises contacting the conditioned breath sample with an interactant that interacts with the ammonia in the conditioned breath sample, wherein the interaction causes a change in an optical characteristic in relation to the amount of the ammonia in the breath sample. It further comprises using the change in the optical characteristic to sense the ammonia in the breath sample. Related apparatuses are also provided.Type: GrantFiled: March 12, 2014Date of Patent: May 7, 2019Assignee: Invoy Holdings, LLCInventors: Brent Satterfield, Lubna Ahmad, Rhett Martineau
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Publication number: 20180318835Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: ApplicationFiled: July 13, 2018Publication date: November 8, 2018Inventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Patent number: 10022718Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: GrantFiled: October 26, 2015Date of Patent: July 17, 2018Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Patent number: 9518991Abstract: Methods and devices are provided for analyzing acetone in breath. One such method comprises disposing a reactant in a reaction zone within the breath analysis device, wherein the reactant comprises a primary amine disposed on a surface, and wherein the reaction zone has an optical characteristic that is at a reference level. It also comprises pre-storing a liquid nitroprusside solution within the breath analysis device separately from the reactant. The method further comprises using the breath analysis device to cause the breath to contact the reactant in the reaction zone so that the acetone in the breath reacts with the reactant to form a reaction product and, after the reaction product has been formed, using the breath analysis device to cause the nitroprusside solution to contact and react with the reaction product and to facilitate a change in the optical characteristic of the reaction zone relative to the reference level.Type: GrantFiled: May 15, 2013Date of Patent: December 13, 2016Assignee: Invoy Technologies, LLCInventors: Lubna M. Ahmad, Brent Satterfield, Rhett Martineau
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Publication number: 20160114323Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: ApplicationFiled: October 26, 2015Publication date: April 28, 2016Inventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Publication number: 20150132858Abstract: Methods and devices are provided for analyzing acetone in breath. One such method comprises disposing a reactant in a reaction zone within the breath analysis device, wherein the reactant comprises a primary amine disposed on a surface, and wherein the reaction zone has an optical characteristic that is at a reference level. It also comprises pre-storing a liquid nitroprusside solution within the breath analysis device separately from the reactant. The method further comprises using the breath analysis device to cause the breath to contact the reactant in the reaction zone so that the acetone in the breath reacts with the reactant to form a reaction product and, after the reaction product has been formed, using the breath analysis device to cause the nitroprusside solution to contact and react with the reaction product and to facilitate a change in the optical characteristic of the reaction zone relative to the reference level.Type: ApplicationFiled: May 15, 2013Publication date: May 14, 2015Inventors: Lubna M. Ahmad, Brent Satterfield, Rhett Martineau
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Publication number: 20140276100Abstract: A system is provided for sensing an analyte in a breath sample. The system includes a breath bag, a cartridge and a base. The breath bag contains the breath sample. The bag includes a mouthpiece fixedly disposed on the breath bag. The cartridge includes an interactant that reacts with the analyte and generates a change in an optical characteristic relative to a reference. The base includes a flow path, a breath bag receiver for detachably receiving and retaining the mouthpiece of the breath bag in fluid communication with the flow path, and a cartridge receiver that detachably receives and retains the cartridge in the base, such that the base engages the cartridge so that the interactant is in fluid communication with the flow path.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Applicant: Invoy TechnologiesInventors: Brent Satterfield, Lubna Ahmad, Rhett Martineau, Zach Smith
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Publication number: 20110275117Abstract: Systems and methods for growing photosynthetic cells that may be used to produce a biomass. The systems and methods recycle liquid and can produce a high cell concentration harvested biomass.Type: ApplicationFiled: October 6, 2009Publication date: November 10, 2011Applicant: ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZ ONA STATE UNIVERSITYInventors: Bruce E. Rittman, Chao Zhou, Raveender Vannela, Mark Holl, Jeff Houkal, Rhett Martineau