Abstract: Multiple digital image tiles are stitched together into a single digital image through use of reactive alpha mask generation, allowing for efficient tile rearrangement and preview capabilities. The present invention features a method for stitching a first image tile and a second image tile to display a preview of a stitched image through use of alpha masking. The method may comprise arranging the first image tile and the second image tile into an initial image display, aligning the first image tile and the second image tile of the initial image display such that shared pixel regions of the first image tile overlap those of the second image tile, stitching the aligned first image tile to the aligned second image tile, applying the alpha blending mask of the stitched image to the initial image display, and displaying the initial image display with the alpha blending mask applied.

Abstract: Systems for allowing adjustable imaging of specimens of various sizes in solutions of various refractive indices, such as those with a refractive index of at least 1.45, for use in microscopes such as fluorescent light sheet microscopes. The systems allow for imaging large specimens in various refractive indices while maintaining the highest optical sectioning provided by the objectives used across the full range of microscope stage travel. The systems also allow the use of a wider range of optics, such as low magnification 2.5× detection objectives, allowing for increased imaging speed and field of view.

Abstract: The present invention features an automated fluid transfer system for tissue incubation. The system comprises a cartridge and a cartridge holder. The cartridge may hold a tissue and comprise a first and second opening to allow a fluid to enter and exit the cartridge. The cartridge holder comprises an inlet and outlet port covered by a first closure mechanism, a drainage port covered by a second closure mechanism, and a sensor. Inserting the cartridge into the cartridge holder may trigger the sensor and activate the first closure mechanism to allow the fluid to enter the sample chamber through the inlet port and for air to escape from the sample chamber through the outlet port. The tissue may incubate in the fluid for a certain amount of time. Once the tissue has finished incubating, the second closure mechanism is actuated and the fluid escapes through the drainage port.

Abstract: Systems for allowing imaging of large specimens in high refractive index solutions, such as those with a refractive index of at least 1.45, for use in microscopes such as fluorescent light sheet microscopes. The systems allow for imaging large specimens in high RI while maintaining the highest optical sectioning provided by the objectives used across the full range of microscope stage travel. The systems also allow the use of a wider range of optics, such as low magnification 2.5× detection objectives, allowing for increased imaging speed and field of view.

Abstract: Systems for allowing adjustable imaging of specimens of various sizes in solutions of various refractive indices, such as those with a refractive index of at least 1.45, for use in microscopes such as fluorescent light sheet microscopes. The systems allow for imaging large specimens in various refractive indices while maintaining the highest optical sectioning provided by the objectives used across the full range of microscope stage travel. The systems also allow the use of a wider range of optics, such as low magnification 2.5× detection objectives, allowing for increased imaging speed and field of view.