Abstract: The invention provides methods for isolating and enhancing the levels of jasmonates from cacao plant sources. In a preferred embodiment, jasmonic acid and 12-hydroxy jasmonate sulfate are detected in various cocoa products, and the levels of these compounds can be manipulated to increase the beneficial health effects of a food product made with the cocoa products The invention includes methods to prepare edible products containing cocoa jasmonates.

Abstract: The invention provides methods for isolating and enhancing the levels of jasmonates from cacao plant sources. In a preferred embodiment, jasmonic acid and 12-hydroxy jasmonate sulfate are detected in various cocoa products, and the levels of these compounds can be manipulated to increase the beneficial health effects of a food product made with the cocoa products The invention includes methods to prepare edible products containing cocoa jasmonates.

Abstract: A high throughput, on-line, pulsed ultrafiltration-mass spectrometric method has been developed to determine whether a compound has predetermined characteristics that would make it suitable for a specific purpose, e.g. drug development. The method is useful to generate, identify, and quantify metabolites of compounds formed by drug metabolizing enzymes such as cytochrome P450, UDP-glucuronyltransferases, and glutathione transferases. The method is useful for rapid screening of drugs or other compounds to determine the extent of their metabolism and to characterize their primary metabolites. If reactive and potentially toxic metabolites are formed during, e.g. cytochrome P450 oxidation, the metabolites can be reacted with glutathione and then detected on-line using mass spectrometry in a rapid assay to assess the potential for toxicity. In addition, the method is useful for the determination of bioavailability, absorption and cell permeability of compounds.

Abstract: A device and method is disclosed for the identification, concentration and isolation of a chemical compound from a mixture of compounds on the basis of the binding affinity of the chemical compound to a macromolecule or complex thereof, that is, to a receptor. Both the compounds to be assayed, and the macromolecules to which the compounds may bind, are free in solution. The method comprises contacting a mixture of compounds in solution with a solution of macromolecules or complexes thereof in a device possessing an ultrafiltration membrane to retain the receptor and bound compound receptor complex; washing through the device non-bound compounds; and releasing a bolus of the bound compound which passes through the ultrafiltration membrane where it may be analyzed chemically and/or spectrally (e.g. mass spectrometry) and/or bioassayed. The device includes a barrier such as an ultrafiltration membrane separating a binding chamber and an effluent chamber.