Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Abstract: The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Abstract: The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: Animal food comprises material which improves and/or optimizes the expression of luminescent proteins in animals. The animals may naturally express luminescent proteins or they may be transgenic animals transfected with a non-endogenous luminescent gene. The animal has a predetermined proteome and the luminescent protein has a predetermined composition of amino acids. The animal food has a base food that is fortified with one or more amino acids which are over-represented in the luminescent protein compared to the amino acids present in the animal proteome. The animal food may further comprise caloric material which increases the cellular metabolism of the animal thereby increasing the expression of the luminescent protein. The animal food may also include inducing material which induces expression of an inducible luminescent gene present in the animal.

Abstract: An aquarium comprises a tank and an excitation light source which is configured to emit light at a wavelength selected to be optimal for causing a transgenic ornamental fish to fluoresce, wherein the ornamental fish expresses one or more fluorescent proteins at a level sufficient such that said fish fluoresces upon exposure to the excitation light source. The aquarium may further comprise one or more of the following: a transgenic fluorescent fish, a second light source, gravel or other ornamental objects, a plant or a water conditioning system. The transgenic fluorescent fish may comprise one or more chimeric fluorescence genes encoding one or more fluorescent proteins.

Abstract: A two piece throttling valve is disclosed. The valve assembly includes a stationary outer housing with an inner removable operable assembly. In this configuration the piping (pump stack) does not have to disassembled to remove, clean or replace the internal valve pieces or the in-line screen. The inner assembly can be quickly removed for cleaning. The entire valve assembly is heated to reduce deposition of volatile process byproducts on the internal valve surfaces and reduce the cleaning frequency. A wedge plate is provided in the outer stationary valve housing to mate with a sloped bottom surface of the inner valve housing such that when the inner assembly is in position in the outer valve housing there is a tight fit between adjacent sealing surfaces around the valve bore. A single flat surface seal provides vacuum sealing between the inner assembly and outer housing. A dual valve stem (shaft) seal seals the valve shaft.