Patents by Inventor Richard K. Wilkosz

Richard K. Wilkosz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method and formulation for stabilization of enzymes
    Patent number: 6294365
    Abstract: The present invention is directed to a method and a formulation for the stabilization of an enzyme for storage at temperatures above freezing. Specifically, the invention involves the use of formulated excipients that when added to an enzyme or mixture of enzymes and subsequently lyophilized renders stability. Upon reconstitution, the stabilized materials are useful in assays, diagnostic or molecular biology research kits, and other biological applications.
    Type: Grant
    Filed: September 30, 1998
    Date of Patent: September 25, 2001
    Assignee: Molecular Biology Resources, Inc.
    Inventors: Therese A. De Rosier, Norberto B. de la Cruz, Richard K. Wilkosz
  • Target nucleic acid sequence amplification
    Patent number: 6063604
    Abstract: A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of those sites, and extensions from those nicks, thereby displacing any existing polynucleotides. Further provided is a method for detecting polynucleotides using the method of the invention. A kit combining the components commonly used in practicing the method of the invention is also provided.
    Type: Grant
    Filed: October 14, 1997
    Date of Patent: May 16, 2000
    Assignee: Molecular Biology Resources, Inc.
    Inventors: James F. Wick, Reinhold Mueller, Michele L. Blassak, Richard K. Wilkosz
  • Method and formulation for stabilization of enzymes
    Patent number: 5876992
    Abstract: The present invention is directed to a method and a formulation for the stabilization of an enzyme for storage at temperatures above freezing. Specifically, the invention involves the use of formulated excipients that when added to an enzyme or mixture of enzymes and subsequently lyophilized renders stability. Upon reconstitution, the stabilized materials are useful in assays, diagnostic or molecular biology research kits, and other biological applications.
    Type: Grant
    Filed: July 3, 1996
    Date of Patent: March 2, 1999
    Assignee: Molecular Biology Resources, Inc.
    Inventors: Therese A. De Rosier, Norberto B. de la Cruz, Richard K. Wilkosz
  • Biologically active fragment of bacillus stearothermophilus DNA polymerase
    Patent number: 5830714
    Abstract: The present invention is directed to an isolated and purified DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Bacillus stearothermophilus. More particularly, the invention is directed to a DNA encoding an approximately 66,000 dalton DNA polymerase that lacks 273 amino acids from the N-terminus of the approximately 96,000 dalton B. stearothermophilus DNA polymerase I, and to the protein encoded thereby which has been designated the B. stearothermophilus DNA polymerase I exo- fragment. The enzyme fragments are useful in DNA sequencing, cDNA preparations, thermophilic Strand Displacement Amplification and other molecular biology applications.
    Type: Grant
    Filed: April 17, 1996
    Date of Patent: November 3, 1998
    Assignee: Molecular Biology Resources, Inc.
    Inventors: Neela Swaminathan, Richard K. Wilkosz